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目的观察马桑内酯致痫及妥泰干扰大鼠中央前回血脑屏障的超微结构改变,探讨癫痫发作及妥泰治疗机制中血脑屏障的意义。方法成年健康雄性SD大鼠30只,随机分为对照组、癫痫组、治疗组;癫痫组、治疗组用马桑内酯注入到大鼠侧脑室,制作癫痫动物模型;实验组用妥泰在癫痫发作后1h灌胃,持续7d,1次/d;7d后取大鼠大脑中央前回,做超薄切片,电镜观察中央前回血脑屏障的超微结构改变。结果癫痫组内皮细胞、基膜、周细胞出现明显水肿,电子密度降低;治疗组水肿缓解。血脑屏障内皮细胞胞质厚度、基膜厚度、周细胞胞质厚度的比较表明,癫痫组与对照组也有显著性差异(P<0.05);治疗组与癫痫组比较,有显著性差异(P<0.05)。结论1.马桑内酯致痫大鼠血脑屏障内皮、基膜、周细胞出现明显水肿,妥泰使水肿缓解;2.血脑屏障超微结构改变可能是癫痫发作及恢复的重要机制。
Objective To observe the changes of ultrastructure of blood-brain barrier (BBB) caused by epilepsy and topiramate induced by docetaxel in rats and to explore the significance of BBB in seizures and topotecan treatment. Methods Thirty adult male Sprague-Dawley rats were randomly divided into control group, epilepsy group and treatment group. Epilepsy group and treatment group were injected intracerebroventricular injection with coriaria lactone to make animal model of epilepsy. The experimental group was treated with topiramate Seizures 1h after intragastric administration, continued 7d, 1 / d; 7d after taking the central front of the brain of the rat, do ultrathin sections, electron microscopy of the central front back to the blood-brain barrier ultrastructural changes. Results Epilepsy group endothelial cells, basement membrane, pericytes obvious edema, electron density decreased; treatment group edema eased. The comparison of cytoplasmic thickness, basement membrane thickness and pericyte cytoplasmic thickness of blood-brain barrier endothelial cells showed that there was also a significant difference between epilepsy group and control group (P <0.05), and there was significant difference between the treatment group and epilepsy group (P <0.05). Ephedra epididymitis induced epilepsy rat blood brain barrier endothelium, basement membrane, pericytes significant edema, Topote edema alleviate; 2. Blood-brain barrier ultrastructural changes may be an important mechanism of seizures and recovery.