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目的:了解珠江三角洲地区酒楼、日韩料理餐厅菜肴所用的海产品和淡水产品中副溶血性弧菌(Vibrio parahae-m olyticus,VP)的污染情况。方法:参照《海产品中副溶血性弧菌的采样及定量检测方案(2004年5月20日)》的生化法、V itek-32鉴定系统、最可能数值(MPN)进行定性及定量;SPSS10.0对数据进行统计分析。结果:103份样品确认VP 51株,阳性率为49.5%。按照地域比较,佛山46.7%,惠州50%,深圳51.7%,增城69.2%,中山58.3%,珠海18%。生吃鱼类阳性率中山最高62.5%,深圳最低12.5%;深圳生吃鱼类MPN浓度几何均值最高(54 MPN/100 g);三文鱼阳性率72.7%(8/11),显著高于其他鱼生(P<0.05)。结论:本次监测结果(51/103份)相比2000年在该地区调查的1.0%(2/196)[3]有显著提高(P<0.05)。高阳性率显示食肆有海产、水产混合养殖的习惯,引至生吃水产品VP污染严重,亟待做好防控工作。
Objective: To investigate the contamination of Vibrio parahae-m olyticus (VP) in marine products and freshwater products used in restaurants in the Pearl River Delta region and in Japanese and Korean restaurants. Methods: According to the biochemical method and Vitek-32 identification system, the most probable value (MPN) was qualitative and quantitative according to the sampling and quantitative detection scheme of Vibrio parahaemolyticus in seafood (May 20, 2004); SPSS10 .0 Perform statistical analysis on the data. Results: There were 103 strains confirmed VP51 strains, the positive rate was 49.5%. According to geographical comparison, Foshan 46.7%, Huizhou 50%, Shenzhen 51.7%, Zengcheng 69.2%, Zhongshan 58.3%, Zhuhai 18%. The positive rate of raw fish was 62.5% in Zhongshan and 12.5% in Shenzhen. The MPN concentration of raw fish was the highest in Shenzhen (54 MPN / 100 g) and salmon was 72.7% (8/11), which was significantly higher than that of other fish Health (P <0.05). Conclusion: The monitoring results (51/103) were significantly higher than those of 1.0% (2/196) [3] surveyed in the area in 2000 (P <0.05). High positive rate shows that restaurants have seafood, aquaculture habits, lead to raw aquatic products VP serious pollution, urgent need to do a good job of prevention and control work.