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目的：了解安氏Ⅱ类错牙合畸形患者矫治前期龈沟液的细菌分布情况，为进一步探讨细菌感染对安氏Ⅱ类错牙合畸形矫治后牙根吸收的影响奠定基础。方法从中南大学口腔医学院收集24例安氏Ⅱ类错牙合畸形的12~18岁患者龈沟液，在厌氧条件下进行细菌培养，PCR扩增细菌基因序列，经测序后通过序列比对鉴定细菌菌株。结果共鉴定出43株菌，其中链球菌属18种菌株，奈瑟菌属7种菌株，放线菌属6种菌株，孪生球菌属2种菌株，罗氏菌属2种菌株，艾肯菌属等其他8个菌属各1种菌株。结论安氏Ⅱ类错牙合畸形患者矫治前期龈沟液中分布多种细菌。“,”Objective To investigate the distribution of bacterial flora in gingival crevicular fluid of AngleII malocclusion orthodontic patients in the early stage of correction, so as to figure out the influence of mi-crobial infection on tooth root absorption in Angle II malocclusion orthodontic patients after correction. Methods The gingival crevicular fluid of 24 Angle II malocclusion orthodontic patients was collected from Xiangya Stomatological Hospital of Central South University. They were 12-18 years old. Then anaerobic cul-tivation was carried out. Bacterial gene sequences were amplified by PCR, and then the bacteria strains were identified by sequence alignment. Results Forty-three strains of bacteria were identified, which included 6 strains of Actinomycetes, 18 strains of Streptococcus, 7 strains of Neisseria, 2 strains of Gemella, and 2 strains of Rothia, 1 strains of Eikenella and other 7 bactaria species. Conclusions There is a wide bacterial spectrum in gingival crevicular fluid of Angle II malocclusion orthodontic patients in the early stage of correction.