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本研究在大鼠胸主动脉内皮损伤内膜增生的模型上观察了内皮损伤后血管反应性的动态变化。实验大鼠随机分为对照组、术后３天组、术后１０天组、术后２１天组４组。组织学检查提示内皮损伤后的大鼠胸主动脉术后２１天内皮尚不能完全修复，且有新生内膜形成。血管条张力测定试验表明，３天组大鼠受损血管对去甲肾上腺素（ＮＥ）的最大收缩反应下降（与对照组比，Ｐ＜０．０００１），对阶梯浓度ＮＥ的反应曲线下移，而１０天组已恢复正常；３天组大鼠受损血管对乙酰胆碱（Ａｃｈ）的最大舒张反应亦显著受损（与对照组比，Ｐ＜０．０００１），对阶梯浓度Ａｃｈ的反应曲线下移，且术后２１天仍不能恢复正常；受损血管对硝普钠介导的非内皮依赖性舒张反应不受影响。提示血管内皮受损可致血管反应性异常，且长时间不能恢复。
In this study, the dynamic changes of vascular reactivity after endothelial injury were observed in a rat model of intimal hyperplasia of the thoracic aorta. The rats were randomly divided into control group, 3 days after operation, 10 days after operation and 4 groups after 21 days. Histological examination suggested that endothelial cells could not be completely repaired within 21 days after thoracic aortic endothelium injury and there was neointimal formation. The vascular tension test showed that the maximal contractile response to norepinephrine (NE) was decreased in the 3-day-old rats (P <0.0001 compared with the control group), and the response to the stepped concentration NE was down , While the 10-day group returned to normal. The maximal relaxation of acetylcholine (Ach) was also impaired significantly in the 3-day-old rats (P <0.0001 compared with the control group), and the response curve to the step concentration Ach Down, and still can not return to normal 21 days after operation; impaired blood vessels on nitroprusside-mediated non-endothelium-dependent relaxation is not affected. Prompted vascular endothelial damage can cause vascular reactivity abnormalities, and can not be restored for a long time.