论文部分内容阅读
目的:从中药斑蝥中制备斑蝥多肽及斑蝥素,并对其抗肿瘤活性进行比较。方法:采用仿生酶解法制备斑蝥多肽,碱性水液超声提取斑蝥素;采用Prestoblue法检验两组物质对人肝癌BEL-7402细胞增殖的抑制作用;检验两组物质对S180荷瘤小鼠的肿瘤、胸腺、脾脏生长的影响。结果:斑蝥多肽和斑蝥素体外能抑制BEL-7402细胞的增殖(P<0.05),斑蝥多肽的抑制率较斑蝥素低12.54%;体内能抑制S180肉瘤的生长(P<0.05),斑蝥素的抑制率较斑蝥多肽高5.93%,且斑蝥多肽对胸腺和脾脏的生长无抑制。结论:斑蝥多肽和斑蝥素均有抗肿瘤活性,但斑蝥多肽无免疫抑制。
OBJECTIVE: To prepare cantharidin peptides and cantharidin from Chinese cantharidin and compare their antitumor activity. Methods: The cantharidin polypeptide was prepared by biomimetic enzymatic method and the cantharidin was extracted by alkaline aqueous solution. Prestoblue method was used to test the inhibitory effect of two substances on the proliferation of human hepatocellular carcinoma BEL-7402 cells. The effects of two substances on tumor of S180 tumor-bearing mice , Thymus, spleen growth. Results: Canthaxanthin and cantharidin inhibited the proliferation of BEL-7402 cells in vitro (P <0.05). The inhibition rate of cantharidin polypeptide was 12.54% lower than that of cantharidin. The growth of S180 sarcoma was inhibited in vivo (P <0.05) The inhibition rate was 5.93% higher than that of Canthaxanthin polypeptide, and Canthaxanthin polypeptide did not inhibit the growth of thymus and spleen. Conclusion: Both cantharidin and cantharidin have anti-tumor activity, but cantharidin polypeptide has no immunosuppression.