Prunella vulgaris L.extract improves cellular immunity in MDR-TB challenged rats

• 作 者: Lu Jun Qin Rui Ye Song Yang Mei
• 机 构: Medical College, Anhui University of Science & Technology, Huainan 232001, China“,”Department of Cli
• 出 处: 中国人民解放军军医大学学报（英文版）
• 发表日期: 2011年4期
• 关键词: Prunella vulgaris L. Extract Multiple drugs resistant bacillus tuberculosi Cytok

Objective:To study the effect of the extract of Prunella vulgaris L.on multiple drugs resistant bacillus tuberculosis (MDR-TB).Methods:Experimental animal model in rats was induced by MDR-TB.Normal group,model group and Prunella vulgaris L.group were set up.The contents of IFN-γ,IL-4,IL-10 and IL-12 were examined by ELISA.Their genome mRNAs were extracted,the target genes were amplified by PCR.RT-PCR was used to detect the mRNA levels of them.Results:The content of IFN-γof the extract of Prunella vulgaris L.group was 1.98+0.67 pg/ml,IL-4 was 6.47+1.46 pg/ml,IL-10 was 12.13+3.43 pg/ml and IL-12 was 3.02+0.86 pg/ml.Compared with the model group,Prunella vulgaris L.group was notable difference in serum IFN-γ,IL-12 and IL-10 (P＜0.05).The mRNA levels of IFN-γ,IL-12 increased and IL-10 decreased obviously,the differences were quite significant (P＜0.05),but IL-4 had no obvious change.Conclusion:The extract of Prunella vulgaris L.can enhance the cellar immunological function in rats from up-regulation of the level of genetic transcription,accordingly provide the theory basis of healing of tuberculosis with it.