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目的探究线粒体融合分裂对新生期反复惊厥海马线粒体凋亡的调节。方法选日龄为6 d的大鼠100只,随机分为腹腔注射NS组,腹腔注射Mdivi组,单纯反复惊厥组,惊厥加Mdivi组。单纯反复惊厥组,惊厥加Mdivi组通过三氟乙脒造反复惊厥模型。检测大鼠脑组织中8-OHd G水平与TUNEL阳性表达情况。结果 NS组,MD组TUNEL阳性率及8-OHd G水平显著低于RS组和RS+MD组(P<0.05);RS+MD组TUNEL阳性率、8-OHd G水平显著低于RS组(P<0.05);注射Mdivi的MD+RS组和MD组的TUNEL阳性率表达及8-OHd G水平则低于未注射Mdivi的NS组和RS组;其中RS组与MD+RS组及NS组存在显著性差异(P<0.05)。结论反复惊厥大鼠脑细胞线粒体分裂而导致细胞凋亡;对线粒体进行保护,抑制其分裂则会使脑细胞凋亡状况减轻。
Objective To investigate the regulation of mitochondrial apoptosis by mitochondrial fusion and division in the hippocampus of newborn infants with recurrent seizures. Methods One hundred and sixty-day-old rats were randomly divided into intraperitoneal injection of NS group, intraperitoneal injection of Mdivi group, recurrent convulsion group, convulsion plus Mdivi group. Recurrent seizure group, convulsion plus Mdivi group repeated intravenous sevoflurane induced convulsion model. The level of 8-OHd G and the positive expression of TUNEL in rat brain were detected. Results The positive rate of TUNEL and the level of 8-OHd G in NS group and MD group were significantly lower than those in RS group and RS + MD group (P <0.05). The positive rates of TUNEL and 8-OHd G in RS + MD group were significantly lower than those in RS group P <0.05). The positive expression of TUNEL and the level of 8-OHd G in Mdivi-treated MD + RS and MD groups were lower than those in Mdivi-treated NS and RS groups There was a significant difference (P <0.05). Conclusion Repeated seizures in rat brain mitochondrial mitochondria led to apoptosis; protection of mitochondria, inhibition of its division will make the brain cells apoptosis reduced.