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目的采用GC-MS法同时测定大鼠血浆中广藿香醇和广藿香酮的含量。方法采用Agilent HP-5ms色谱柱(30 m×0.25 mm,0.25μm),电子碰撞离子源(EI),电子倍增电压(EMV)模式,检测离子分别为:广藿香醇m/z 222.2、广藿香酮m/z 168.0。结果广藿香醇5.040~2016 ng·m L-1(r=0.9979)、广藿香酮1.020~406.0 ng·m L-1(r=0.9958)与峰面积比值的线性关系良好;批内、批间的方法回收率均<±15%,批内、批间精密度的RSD均<8%;平均萃取回收率分别为76.4%、80.6%。结论所用方法简单、灵敏、特异性高、定量准确,可为大鼠血浆中广藿香醇和广藿香酮的药动学研究提供参考。
Objective To determine the contents of patchouli alcohol and patchoulenone in rat plasma by GC-MS simultaneously. Methods Agilent HP-5ms column (30 m × 0.25 mm, 0.25 μm), electron impact ionization (EI) and electron multiplied voltage (EMV) mode were used. The detection ions were patchouli alcohol m / z 222.2, patchouli Fragrance ketone m / z 168.0. Results Patchouli alcohol 5.040 ~ 2016 ng · m L-1 (r = 0.9979), patchouli ketone 1.020 ~ 406.0 ng · m L-1 (r = 0.9958) had a good linear relationship with the peak area ratio. The recoveries were between ± 15%. The RSDs of intra-batch and inter-batch precision were all less than 8%. The average extraction recoveries were 76.4% and 80.6%, respectively. Conclusion The method is simple, sensitive, specific and accurate. It can provide a reference for pharmacokinetics study of patchoulen and patchoulenone in rat plasma.