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普通洋葱(Allium cepa L.)是一种开放授粉的重要蔬菜作物,在洋葱品种的纯度鉴定中因其亲本保有一定程度的异质性,所以杂交种的纯度百分率难以确定.为了有效地控制杂交种的纯度质量,本研究从收集于各文献的89对引物中筛选出了12对具有多态性的引物,利用筛选出的12对SSR引物对39份洋葱种质进行分析,检测到47条多态性带,平均有效等位基因数为7.25.可变类平均法(Flexible group linkage)聚类分析表明,SSR标记可将39份洋葱划分为三大类,聚类分析结果与洋葱的自然属性是相符合的.同时发现这些标记在常规品种、亲本自交系内,甚至在杂种一代中都显示出很高的遗传异质性.为了促进和提升繁育品种质量鉴定的准确性,本研究所提出的SSR分子标记辅助育种方案,可通过选定的SSR位点纯和的原种田,制出杂交种.该方案能在选育的几个SSR位点检测出准确的杂交率,还可以有效地监测洋葱F1代杂交种的纯度质量,并且这个方案可以用于任何表现出严重的近交衰退的异花授粉作物的SSR标记分子纯度鉴定,为促进和提升繁育品种质量鉴定的准确性提供帮助.“,”Allium cepa (L.) is an important vegetable crop in open pollination.It is difficult to determine the purity percentage of hybrids their parents have a certain degree of heterogeneity.In order to effectively control the purity quality of hybrids, 12 pairs of polymorphic primers were screened from 89 pairs of primers collected from each literature in this study.39 onion germplasms were analyzed with 12 pairs of SSR primers, and 47 polymorphic bands were detected, with the average number of effective alleles of 7.25.Flexible group linkage clustering analysis showed that SSR markers could divide 39 onions into three categories, which were consistent with the natural attributes of onion.At the same time, these markers showed high genetic heterogeneity in conventional varieties, parental inbred lines, and even in theIn order to promote and enhance the accuracy of the quality identification of breeding varieties, the SSR molecular marker assisted breeding scheme proposed in this study could be used to produce hybrids through selected SSR site pure and original seed fields.This method could detect the accurate hybridization rate at several SSR loci selected, and also could effectively monitor the purity and quality ofIn addition, this scheme could be used to identify the purity of SSR markers in any non flower pollinated crops showing severe inbreeding, and to help promote and enhance the accuracy of the quality identification of breeding varieties.