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本文采用的人B淋巴母细胞株(Ramos,6-TG~(?))由上海细胞生物研究所惠赠,其EB病毒核抗原(EBNA)阴性。我们将该株用N-基-N-硝基-亚硝基胍(MNNG)(2μg/ml)诱变,和6-硫代鸟嘌呤(6-TG)(20μg/ml)筛选,获得抗高浓度6
The human B lymphoblastoid cell line (Ramos, 6-TG?) Used in this paper is a kind gift from Shanghai Institute of Cell Biology and its negative for Epstein-Barr virus nuclear antigen (EBNA). We screened the strain with N-N-nitro-nitrosoguanidine (MNNG) (2 μg / ml) and 6-thioguanine (6-TG) High concentration 6