Folic Acid Modified Electrospun Poly(vinyl alcohol)/Polyethyleneimine Nanofibers for Cancer Cell Cap

• 作 者: Zhang-yu Fan Yi-li Zhao 朱晓玥 Yu Luo Ming-wu Shen 史向阳
• 机 构: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University,Key Laboratory of T
• 出 处: Chinese Journal of Polymer Science
• 发表日期: 2016年06期
• 关键词: alcohol vinyl capture neoplasm glycol qualitative metastatic vinyl alcohol captu

Capture and detection of metastatic cancer cells are crucial for diagnosis and treatment of malignant neoplasm.Here, we report the use of folic acid(FA) modified electrospun poly(vinyl alcohol)(PVA)/polyethyleneimine(PEI)nanofibers for cancer cell capture applications. Electrospun PVA/PEI nanofibers crosslinked by glutaraldehyde vapor were modified with FA via a poly(ethylene glycol)(PEG) spacer, followed by acetylation of the fiber surface PEI amines. The formed FA-modified nanofibers were well characterized. The morphology of the electrospun PVA/PEI nanofibers is smooth and uniform despite the surface modification. In addition, the FA-modified nanofibers display good hemocompatibility as confirmed by hemolysis assay. Importantly, the developed FA-modified nanofibers are able to specifically capture cancer cells overexpressing FA receptors, which were validated by quantitative cell counting assay and qualitative confocal microscopy analysis. The developed FA-modified PVA/PEI nanofibers may be used for capturing circulating tumor cells for cancer diagnosis applications.

Capture and detection of metastatic cancer cells are crucial for diagnosis and treatment of malignant neoplasm. Here, we report the use of folic acid (FA) modified electrospun poly (vinyl alcohol) (PVA) / polyethyleneimine (PEI) nanofibers for cancer cell capture applications . Electrospun PVA / PEI nanofibers crosslinked by glutaraldehyde vapor were modified with FA via a poly (ethylene glycol) (PEG) spacer, followed by acetylation of the fiber surface PEI amines. The morphology of the In addition, the FA-modified nanofibers display confirmed hemocompatibility as confirmed by hemolysis assay. Importantly, the developed FA-modified nanofibers are able to specifically capture cancer cells overexpressing FA receptors, which were validated by quantitative cell counting assay and qualitative confocal microscopy analysis. The developed FA-modified PVA / PEI nanofiber s may be used for capturing circulating tumor cells for cancer diagnosis applications.