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选用30 nm和50 nm羧基化聚苯乙烯微球(COOH-PBs)作用于RAW264.7巨噬细胞,用真空紫外圆二色光谱(SRCD)分析细胞DNA二级结构的可能变化,并用彗星电泳验证此法的可行性和优越性。研究结果显示,两种颗粒都致使DNA螺旋度降低及单链程度增加,30 nm COOH-PBs造成的损伤甚于50 nm,表明纳米颗粒影响DNA的稳定性有粒径依赖性。SRCD为深入研究纳米颗粒对细胞DNA稳定性的影响及其可能原因提供了一个可行的研究方法和途径。
The macrophages of RAW264.7 cells were treated with 30 nm and 50 nm carboxylated polystyrene microspheres (COOH-PBs), and the possible changes of DNA secondary structure were analyzed by vacuum ultraviolet circular dichroism spectroscopy (SRCD) Verify the feasibility and superiority of this method. The results showed that both of the two kinds of particles caused the DNA helicity to decrease and the degree of single strand to increase, and the damage caused by COOH-PBs at 30 nm was more than 50 nm, indicating the particle size dependence of nanoparticle’s influence on DNA stability. SRCD provides a feasible method and approach for further study of the effect of nanoparticles on cell DNA stability and its possible causes.