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目的:探讨人食管癌EC9706细胞中胰岛素样生长因子1受体的表达,及其对反义寡核苷酸胰岛素样生长因子1受体mRNA表达的影响和食管癌发生、转移的意义。方法:实验于2004-09/12在河南省肿瘤病理重点实验室完成。体外分5组培养人食管癌EC9706细胞,其中4组分别用设计合成的3条封闭胰岛素样生长因子1受体不同基因位点的反义寡核苷酸1～3及1条无关寡核苷酸转染,另一组不转染。采用完整细胞原位杂交技术观察各组EC9706细胞中胰岛素样生长因子1受体mRNA表达情况。结果:①胰岛素样生长因子1受体mRNA在食管癌EC9706细胞中呈阳性表达。②3条胰岛素样生长因子1受体反义寡核苷酸转染组EC9706细胞中胰岛素样生长因子1受体mRNA的表达无差异(2.66±0.21,2.63±0.23,2.65±0.22,P>0.05),但均显著低于无关寡核苷酸组及无转染组(5.67±2.86,5.02±2.75,P<0.05)。结论:胰岛素样生长因子1受体反义寡核苷酸转染人食管癌EC9706细胞后,可体外抑制EC9706细胞中胰岛素样生长因子1受体mRNA的表达,从而抑制食管癌的发生和转移。为临床预防食管癌的发生、发展和转移提供了理论依据。
Objective: To investigate the expression of insulin-like growth factor-1 receptor in human esophageal carcinoma EC9706 cells and its effect on the expression of antisense oligonucleotide insulin-like growth factor 1 receptor mRNA and the occurrence and metastasis of esophageal carcinoma. Methods: The experiment was performed at Henan Key Laboratory of Pathology from September to December 2004. In vitro, EC9706 cells were cultured in 5 groups, of which 4 were treated with 3 synthetic antisense oligonucleotides, 1 to 3, and 1 unrelated oligonucleotide, respectively, which blocked the insulin-like growth factor 1 receptor Acid transfection, the other group did not transfected. Whole cell in situ hybridization was used to observe the expression of insulin-like growth factor 1 receptor mRNA in EC9706 cells. Results: ① IGF-1 mRNA was positively expressed in EC9706 cells. ② The expression of insulin-like growth factor 1 receptor mRNA in EC9706 cells transfected with 3 insulin-like growth factor 1 receptor antisense oligonucleotide did not differ significantly (2.66 ± 0.21, 2.63 ± 0.23, 2.65 ± 0.22, P> 0.05) , But both of them were significantly lower than that of unrelated oligonucleotide group and non-transfected group (5.67 ± 2.86,5.02 ± 2.75, P <0.05). CONCLUSION: Transfection of insulin like growth factor 1 receptor antisense oligonucleotide into human esophageal carcinoma EC9706 cells can inhibit the expression of insulin-like growth factor 1 receptor mRNA in EC9706 cells and inhibit the occurrence and metastasis of esophageal carcinoma in vitro. It provides a theoretical basis for clinical prevention of the occurrence, development and metastasis of esophageal cancer.