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目的:采用HPLC法测定臁疮丸中绿原酸的含量。方法:采用Agilent ZORBAX SB-C_(18)色谱柱;以乙腈-0.4%磷酸溶液(13:87)为流动相;检测波长327nm;流速为1.0ml·min~(-1)。结果:绿原酸进样量在0.2016～2.0160μg范围内呈现良好的线性关系(r=0.9999),平均回收率为99.76%,RSD为0.99%。结论:该方法快捷、简便、准确,可作为该制剂的质量控制。
Objective: To determine the content of chlorogenic acid in acne bolus by HPLC. Methods: Agilent ZORBAX SB-C_(18) column was used. The mobile phase consisted of acetonitrile-0.4% phosphoric acid solution (13:87). The detection wavelength was 327 nm and the flow rate was 1.0 ml·min~(-1). RESULTS: The chlorogenic acid injection showed a good linear relationship in the range of 0.2016-2.0160 μg (r=0.9999). The average recovery was 99.76% and the RSD was 0.99%. Conclusion: This method is quick, simple, accurate and can be used as the quality control of the preparation.