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目的探讨心肌缺血预适应家兔血浆内皮素(ET)及血清一氧化氮(NO)水平的变化,探讨其在心肌缺血预适应过程中的作用。方法16只新西兰大耳白兔随机分为2组。缺血再灌注组(I/R组):结扎其冠状动脉左室支40min后再灌注120min;缺血预适应组(IP组):先予缺血预适应的过程,结扎冠状动脉左室支5min,放松冠脉血管后再灌注5min,反复操作3次,再按照I/R组进行操作。实验开始前取血测定血清CK、LDH、NO及血浆ET水平,2组动物均在结扎左室支40min、再灌注120min复测NO及ET水平,观察NO和ET变化。结果IP组阻断40min及再灌注120min血浆ET水平及LDH升高低于I/R组,二组比较差异有统计学意义(Pa<0.01),血清NO水平升高多于I/R组,二组比较差异有统计学意义(P<0.01)。IP组再灌注120min,CK水平升高于I/R组,心肌损伤程度小于I/R组。结论心肌缺血预适应家兔NO及ET水平发生明显变化,缺血预适应过程中NO水平升高增强了心肌对缺血的耐受性,ET水平降低减少了心肌缺血过程中其对心肌的损伤。
Objective To investigate the changes of plasma endothelin (ET) and serum nitric oxide (NO) in myocardial ischemic preconditioning rabbits to explore its role in myocardial ischemic preconditioning. Methods Sixteen New Zealand white rabbits were randomly divided into two groups. Ischemia / reperfusion group (I / R group): Ligation of the coronary artery left ventricular branch 40min and then reperfusion for 120min; ischemic preconditioning group (IP group): pre-ischemic preconditioning process, ligation of left ventricular coronary artery 5min, coronary artery relaxation after reperfusion 5min, repeated 3 times, and then follow the I / R group to operate. The levels of serum CK, LDH, NO and plasma ET were measured before the start of the experiment. The levels of NO and ET in the two groups were measured by ligating the left ventricular branch for 40 minutes and reperfusion for 120 minutes. Results Compared with I / R group, the level of plasma ET and the level of LDH in the IP group blocked for 40 minutes and 120 minutes after reperfusion were significantly lower than those in the I / R group (P <0.01) The difference was statistically significant (P <0.01). IP group reperfusion 120min, CK levels increased in the I / R group, myocardial injury was less than the I / R group. Conclusions There was a significant change of NO and ET levels in ischemic preconditioning rabbits. Elevated NO levels in ischemic preconditioning increased myocardial tolerance to ischemia, and decreased ET levels reduced myocardial ischemia Damage.