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In this report,the β-glucosidase from the C.thermocopriae JT3-3 strain was studied.By purifying,the enzyme specific activity was increased about 30 times,and t
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In this report,the β-glucosidase from the C.thermocopriae JT3-3 strain was studied.By purifying,the enzyme specific activity was increased about 30 times,and the yield was about 2%.The molecular weight of β-glucosidase is 50000 by gel filtration chromatography,and about 46000 by SDS polyacrylamide eIectrophoresis.Next the effects of pH and temperature on enzyme activity were studied and the Km value for β-glucosidase was calculated from Lineweaver-Burk.In addition,we succeeded in the cloning and expression of β-glucosidase gene from C.thermocopriae to E.coli cells using pBR322 as a vector.
In this report, the β-glucosidase from the C.thermocopriae JT3-3 strain was studied.By purifying, the enzyme specific activity was increased about 30 times, and the yield was about 2%. The molecular weight of β-glucosidase is 50000 by gel filtration chromatography, and about 46000 by SDS polyacrylamide eIectrophoresis .Next the effects of pH and temperature on enzyme activity were studied and the Km value for β-glucosidase was calculated from Lineweaver-Burk. addition, we succeeded in the cloning and expression of β-glucosidase gene from C. thermocopriae to E. coli cells using pBR322 as a vector.
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