论文部分内容阅读
目的：探讨反复呼吸道感染（ＲＲＴＩ）患儿Ｔ细胞增殖（ＴＬＰ）及其胞浆游离钙浓度（〔Ｃａ２＋〕ｉ）的关系。方法：采用ＭＴＴ比色法测定３０例ＲＲＴＩ患儿及３１名健康儿童ＴＬＰ，使用Ｆｕｒａ－２／Ａｍ做荧光探剂，双波长荧光分光光度仪测定Ｔ细胞活化及未活化时〔Ｃａ２＋〕ｉ。结果：ＲＲＴＩ患儿ＴＬＰ明显低于对照组，Ｔ细胞未活化时两组间〔Ｃａ２＋〕ｉ的差异无统计学意义，当Ｔ细胞活化时ＲＲＴＩ患儿〔Ｃａ２＋〕ｉ明显低于对照组。结论：ＲＲＴＩ患儿ＴＬＰ降低，Ｔ细胞活化过程中〔Ｃａ２＋〕ｉ的动员存在一定程度异常，这是其ＴＬＰ降低的原因之一。
Objective: To investigate the relationship between T cell proliferation (TLP) and cytoplasmic free calcium concentration (〔Ca2 +〕 i) in children with recurrent respiratory tract infection (RRTI). Methods: MTT assay was used to determine TLP in 30 RRTI children and 31 healthy children. Fura-2 / Am was used as a fluorescent probe and dual-wavelength fluorescence spectrophotometer was used to measure the [Ca2 +] i of T cells during activation and deactivation. Results: The TLP in children with RRTI was significantly lower than that in the control group. There was no significant difference of [Ca2 +] i between the two groups when T cells were not activated. [Ca2 +] i in RRTI children was significantly lower than that in control group when T cells were activated. CONCLUSIONS: TLR in children with RRTI is decreased, and mobilization of [Ca2 +] i during T cell activation is somewhat abnormal, which is one of the reasons for the decrease of TLP.