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目的:通过搭建超声神经调控系统，并对超声换能器的声场进行表征，观察超声神经调控技术对大脑中动脉闭塞(MCAO)小鼠模型的干预作用。方法:39只MCAO模型小鼠分为三组：MCAO组(13只)、假刺激组(13只)和超声刺激组(13只)。超声刺激组将中心频率为500 kHz的超声换能器作用于MCAO模型小鼠的脑梗死半球(本研究中为左侧)，每次刺激时间为15 min，连续刺激5 d。在假刺激组中，将同样的超声换能器作用于脑梗死半球，但不打开功率放大器。在超声刺激后的第1 d、第3 d和第5 d进行TTC染色和旷场实验以评估小鼠的脑缺血体积和运动能力。结果:与MCAO组和假刺激组相比，在超声刺激后的第3 d，超声刺激组模型小鼠的脑梗死体积占比百分数明显减少[MCAO组(23.45±1.99)%，假刺激组(22.43±3.07)%，超声刺激组(8.53±3.43)%，n P<0.01]，同时在超声刺激后第5 d观察到小鼠的运动总距离增加，但差异无统计学意义[MCAO组(1 113.04±206.30)cm，假刺激组(944.08±206.60)cm，超声刺激组(1 629.45±54.10)cm，n P＝0.07]。n 结论:低强度聚焦超声作为一种神经调控技术可以显著减少缺血性脑卒中的脑损伤，同时对损伤后运动功能的恢复有一定的促进作用，因此在缺血性脑卒中的干预上有很大潜力。“,”Objective:To study the effect of ultrasound neuromodulation technology on middle cerebral artery occlusion (MCAO) mouse model through the establishment of an ultrasound neuromodulation system and the characterization of the acoustic field of ultrasound transducer.Methods:Tirty-nine MCAO model mice were divided into three groups: MCAO group (13 mice), sham stimulation group (13 mice) and ultrasound stimulation group (13 mice). The ultrasound with a center frequency of 500 kHz was applied to the cerebral infarction hemisphere of MCAO model mice (left side in this study) in ultrasound stimulation group. The duration of ultrasound stimulation was 15min, and stimulation was done for 5 days. In the sham stimulation group, the same ultrasonic transducer was applied to the infarcted hemisphere without turning on the power amplifier. TTC staining and open field were performed on the first, third, and fifth days after ultrasound stimulation to assess cerebral ischemia volume and mobility of the mice.Results:The percentage of cerebral infarction volume of the ultrasound stimulation group (8.53±3.43)% was significantly reduced on the third day after ultrasound stimulation compared with the MCAO group (23.45±1.99)% and the sham stimulation group (22.43±3.07)%(n P<0.01). The mobility of the mice was improved on the fifth day after ultrasound stimulation, but there was no statistical difference among the three groups[MCAO group (1 113.04±206.30)cm, sham stimulation group (944.08±206.60)cm, ultrasound stimulation group (1 629.45±54.10)cm,n P=0.068].n Conclusions:Low intensity focused ultrasound, as a neuromodulation technology, can reduce the brain damage of ischemic stroke and promote the recovery of motor function, and has great potential for the intervention of ischemic stroke.