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目的:运用统计学方法,初步探讨黄连解毒汤提取动态过程中“沉淀物”的产生及机制。方法:采用UV测定其中总生物碱、总黄酮的量,HPLC同时测定小檗碱、药根碱、巴马汀、黄芩苷、栀子苷的量并计算固含率,沉淀率。采用SPSS统计软件评价各指标性成分、沉淀率、固含率之间的相关性。结果:总生物碱中的小檗碱等的量均与总生物碱呈较好的相关性。总黄酮中黄芩苷与总黄酮的相关系数并不高,而总黄酮与栀子苷的相关系数略高;沉淀率与各指标性成分不显示有极高的相关性。固含率与各指标性成分的相关系数相对较大。结论:随提取时间的变化,提取液中以小檗碱为代表的总生物碱及黄芩苷等达到一定浓度后可视为一平衡点或称为沉淀生成的临界点,即可发生反应,生成“沉淀物”;由于“沉淀物”的生成,降低了上述成分浓度,破坏了“平衡状态”。药材中相关成分溶出速度增加,同时沉淀物可能部分溶解,达到新的平衡状态。
Objective: To investigate the production and mechanism of “sediment ” during the dynamic process of extracting Huanglian Jiedu Decoction by using statistical methods. Methods: The amount of total alkaloids and total flavonoids were determined by UV. The contents of berberine, jatrorrhizine, palmatine, baicalin and geniposide were determined simultaneously by HPLC and the solid content and deposition rate were calculated. SPSS statistical software was used to evaluate the correlation between the index components, precipitation rate and solid content. Results: The amount of total alkaloids such as berberine and total alkaloids showed a good correlation. The correlation coefficient between baicalin and total flavonoids in total flavonoids was not high, while the correlation coefficient between total flavonoids and geniposide was slightly higher. The precipitation rate did not show a very high correlation with each index component. The correlation coefficient between the solid content and each index component is relatively large. Conclusion: With the change of extraction time, the total alkaloids and baicalin represented by berberine in the extract can reach a certain concentration and can be regarded as a balance point or the critical point of precipitation, “Sediment ”; due to “sediment ” generation, reducing the concentration of the above ingredients, undermining the “equilibrium state ”. Relevant ingredients dissolution rate of herbs increased, while the precipitate may be partially dissolved, reaching a new equilibrium.