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本实验用溴化二甲噻唑二苯四氮唑（ＭＴＴ）染色，酶标仪测定人胃低分化粘液腺癌ＭＧｃ８０－３及其克隆株体外增殖力，用改良的双层琼脂法测定其克隆形成率。用体外超声粉碎机粉碎脐带静脉内皮细胞及内皮下基质后包被９６孔板、作为人造的血管壁，然后用酶标仪定量分析ＭＧｃ８０－３及其克隆株的粘附力。用扫描电镜观察肿瘤细胞粘附血管壁的形态学过程。结果显示：ＭＧｃ８０－３及其克隆株之间体外的增殖力不同。其中Ｃ１１体外增殖力最强，Ｃ３最弱；Ｃ１１干细胞数最多，Ｃ５干细胞数最少。ＭＧｃ８０－３及其克隆株Ｃ２、Ｃ３、Ｃ１１的粘附力有明显不同。其中３０ｍｉｎ时Ｃ２的粘附力最高，Ｃ５最低；６０ｍｉｎ、９０ｍｉｎ、１２０ｍｉｎ时Ｃ１１粘附力最高，Ｃ５最低。扫描电镜观察ＭＧｃ８０－３对血管内皮粘附的形态学改变，显示３０ｍｉｎ时大部分癌细胞开始粘附于内皮细胞的表面，伸出丝状伪足附着在管腔内表面，癌细胞多数是几个一起粘附；随时间延长粘附癌细胞的数量增多，癌细胞附着处内皮细胞收缩，其间的基质被粘附的癌细胞溶解形成沟壑。结果提示：同一母系及不同克隆株之间增殖力、粘附力存在异质性，增殖力、粘附力之间密切相关。另外，本实验用定量方法筛选出粘附能力较强克隆?
In this experiment, the proliferative capacity of human gastric poorly differentiated mucinous adenocarcinoma MGc80-3 and its clonal strain was measured by MTT assay, and the clones were assayed by a modified double-layered agar assay. Formation rate. After smashing the umbilical vein endothelial cells and the subendothelial matrix with an in vitro ultrasonic disintegrator, 96-well plates were coated and used as artificial blood vessel walls, and then the adhesion of MGc80-3 and its clones was quantitatively analyzed using a microplate reader. Scanning electron microscopy was used to observe the morphological process of tumor cells adhering to the vessel wall. The results showed that the in vitro proliferation of MGc80-3 and its clones was different. Among them, C11 had the strongest proliferative capacity in vitro, and C3 was the weakest. The number of C11 stem cells was the most and the number of C5 stem cells was the least. The adhesion of MGc80-3 and its clones C2, C3, and C11 were significantly different. Among them, the adhesion force of C2 was the highest at 30 minutes, the lowest was at C5; the adhesion force of C11 was the highest and the C5 was lowest at 60 minutes, 90 minutes, and 120 minutes. The morphological changes of MGc80-3 on vascular endothelium adhesion were observed by scanning electron microscope. It showed that most of the cancer cells began to adhere to the surface of endothelial cells at 30 minutes, and the filopodia was attached to the inner surface of the lumen. Most of the cancer cells were Adhesion together; over time, the number of adherent cancer cells increases, the endothelial cells at the attachment site of the cancer cells contract, and the adhering cancer cells dissolve and form sulci. The results suggest that there is heterogeneity in proliferation and adhesion between the same mother and different clones, and the prolificacy and adhesion are closely related. In addition, this experiment used a quantitative method to screen out strong adhesion clones?