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目的:探讨静脉输注体外培养扩增的内皮祖细胞(EPC)对急性心肌梗死大鼠的血管新生、心肌梗死面积以及心功能的影响。方法:分离大鼠骨髓单个核细胞,EBM-2+Single Quots培养基培养,鉴定EPC表面标志,EGFP-Ad标记。建立大鼠急性心肌梗死模型,在心梗后24 h,将体外培养的EPC经尾静脉缓慢注入动物体内。分别在细胞输注后7,14,28 d,检测输注细胞在心肌梗死周边区的分布、新生血管密度、心梗面积以及心功能等多项指标。结果:细胞输注后第7天,EPC治疗组心梗面积(25.08±1.18)%,明显低于对照组的(32.63±1.16)%;细胞输注后第14天时,EPC治疗组梗死周边区可见大量EGFP阳性细胞,并形成管腔样结构,EPC治疗组血管密度为(14.36±1.48)/mm2,明显高于对照组的(6.33±0.69)/mm2;细胞输注第28天,EPC治疗组大鼠各项心功能指标均明显优于对照组。结论:静脉输注的EPC能有效的向心肌梗死周边区募集,形成新生血管,减少心肌梗死面积,促进心功能的恢复。
Objective: To investigate the effects of endothelial progenitor cells (EPCs) cultured in vitro on the angiogenesis, myocardial infarct size and cardiac function in acute myocardial infarction rats. METHODS: Rat bone marrow mononuclear cells were isolated and cultured in EBM-2 + Single Quots medium to identify EPC surface markers and EGFP-Ad markers. A rat model of acute myocardial infarction was established. EPCs cultured in vitro were slowly injected into the animals via caudal vein 24 h after myocardial infarction. The number of transfused cells in peripheral zone of myocardial infarction, neovascular density, myocardial infarct size and cardiac function were measured at 7, 14 and 28 days after cell infusion, respectively. Results: On the 7th day after the cell infusion, the myocardial infarction area in the EPC-treated group (25.08 ± 1.18)% was significantly lower than that in the control group (32.63 ± 1.16)%. On the 14th day after the cell infusion, A large number of EGFP positive cells were observed and the lumen-like structure was formed. The EPC density in the EPC-treated group was (14.36 ± 1.48) / mm2, which was significantly higher than that in the control group (6.33 ± 0.69) / mm2. EPCs The indexes of cardiac function in group rats were obviously better than those in control group. Conclusion: Intravenous EPC can effectively recruit peripheral areas of myocardial infarction, form new blood vessels, reduce the area of myocardial infarction and promote the recovery of cardiac function.