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在研制用于对厚的生物样品进行光学断层成像的共焦扫描荧光显微镜时 ,由于成像信号十分微弱及存在很强的多次散射作用 ,因此杂散光的抑制非常重要 ,而信噪比、信号背景比就成为决定能否获得高对比度、高分率图像的关键。运用光学信息量的概念 ,在已有的光学成像系统信息量计算、共焦扫描荧光显微镜信噪比及传递函数计算的基础上 ,详细分析了共焦扫描荧光显微镜信息量与信噪比等之间的定量关系。该关系表明 ,为了充分利用共焦扫描荧光显微镜的成像性能 ,必须选择适当的探测小孔。所得的结果对于共焦扫描荧光显微成像系统的研制有重要的实用价值
In the development of confocal scanning fluorescence microscopy for optical tomography of thick biological samples, the suppression of stray light is very important due to the very weak imaging signal and the strong multiple scattering effect, and the signal to noise ratio, the signal Background ratio has become the key to decide whether to get high contrast, high score images. Using the concept of optical information volume, the information amount of confocal scanning fluorescence microscope and the signal-to-noise ratio The quantitative relationship between. This relationship shows that in order to take full advantage of the imaging performance of a confocal scanning fluorescence microscope, appropriate detection of the wells must be selected. The results obtained for confocal scanning fluorescence microscopy imaging system has important practical value