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以2种配体去甲基斑蝥酸钠(Na2DCA=7-氧杂二环[2.2.1]庚烷-2,3-二甲酸钠)和咪唑(IM),分别与镍(Ⅱ)和镉(Ⅱ)的醋酸盐通过溶液法合成了2种配合物[Ni(IM)(DCA)(H2O)2]·2H2O(1),[Cd2(IM)4(DCA)2]·2H2O(2)。应用元素分析、热重分析、红外光谱及X-射线单晶衍射法对配合物的组成和结构进行了表征。配合物1与2的中心离子分别与咪唑的亚胺氮原子、去甲基斑蝥酸根的羧基氧原子和醚键氧原子配位,配位数均为6,分别为单核(1)和双核(2)配合物。通过紫外光谱法、荧光光谱法和粘度法研究了配合物与DNA的相互作用。结果表明,配合物能通过部分插入模式与DNA发生较强的结合作用(Kb:5.51×103(1)、1.01×103(2)L·mol-1)。同时,利用荧光光谱法研究了配合物与牛血清白蛋白(BSA)的相互作用。配合物能与BSA发生强烈的相互作用(KA:1.91×105(1)和6.17×105(2)L·mol-1),结合位点数均为1。测试了配合物对人肝癌细胞(SMMC7721)和人乳腺癌(MCF-7)的体外抗增殖活性。结果显示,配合物对不同癌细胞具有选择性抑制作用。镍配合物(1)对SMMC7721的抗癌活性较去甲基斑蝥酸钠有明显提高。
Two ligands, sodium cresylate (Na2DCA = sodium 7-oxabicyclo [2.2.1] heptane-2,3-dicarboxylate) and imidazole (IM) (DCA) (H2O) 2] · 2H2O (1), [Cd2 (IM) 4 (DCA) 2] · 2H2O (2) . The composition and structure of the complex were characterized by elemental analysis, thermogravimetric analysis, infrared spectroscopy and X-ray single crystal diffraction. The central ions of complexes 1 and 2 are coordinated with the imine nitrogen atom of imidazole and the carboxyl oxygen atom and the ether bond oxygen atom of desmethyl cantharidate respectively. The coordination number is 6, which are mononuclear (1) and binuclear (2) complex. The interaction between the complex and DNA was studied by UV spectroscopy, fluorescence spectroscopy and viscometry. The results showed that the complexes could strongly bind DNA with partial insertion patterns (Kb: 5.51 × 103 (1), 1.01 × 103 (2) L · mol-1). At the same time, the interaction of the complex with bovine serum albumin (BSA) was studied by fluorescence spectroscopy. The complexes can strongly interact with BSA (KA: 1.91 × 105 (1) and 6.17 × 105 (2) L · mol-1), respectively. The in vitro anti-proliferative activity of the complex on human hepatocellular carcinoma cells (SMMC7721) and human breast cancer (MCF-7) was tested. The results show that the complexes have selective inhibitory effect on different cancer cells. The anticancer activity of nickel complex (1) on SMMC7721 was significantly higher than that of sodium demethyl cantharidate.