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目的探讨幽门螺杆菌(H.pylori)感染与分泌型卷曲相关蛋白(SFRPs)基因启动子甲基化的关系。方法随机抽取河北医科大学第四医院2012年10—11月内镜咬检不同胃黏膜病变标本72例,其中胃癌40例,异型增生17例,慢性胃炎15例。采用甲基化特异性聚合酶链反应(MSP)技术检测72例胃黏膜组织中SFRPs基因启动子甲基化水平,并应用免疫组化法检测H.pylori感染情况。结果 72例标本中,测得SFRP1、SFRP2、SFRP4、SFRP5基因启动子甲基化分别为34例(47.2%)、36例(50.0%)、27例(37.5%)和30例(41.7%)。SFRP1、SFRP2、SFRP4、SFRP5基因启动子甲基化率:异型增生组〔29.4%(5/17)、35.3%(6/17)、11.8%(2/17)、23.5%(4/17)〕和胃癌组〔72.5%(29/40)、75.0%(30/40)、62.5%(25/40)、65.0%(26/40)〕均高于慢性胃炎组(均为0;P<0.05);胃癌组又高于异型增生组(P<0.05)。72例标本中H.pylori感染率为30.6%(22/72),其中异型增生组、慢性胃炎组H.pylori感染率〔76.5%(13/17)和33.3%(5/15)〕均高于胃癌组〔10.0%(4/40)〕(P<0.05),异型增生组又高于慢性胃炎组(P<0.05)。Spearman等级相关分析显示,H.pylori感染与SFRPs基因启动子甲基化无线性相关(r s=0.264,P>0.05)。结论 SFRPs基因启动子甲基化是胃癌发生的重要因素之一;H.pylori感染也是胃癌发生发展中的重要因素,但是H.pylori感染与SFRPs基因启动子甲基化无关。
Objective To investigate the relationship between Helicobacter pylori (H.pylori) infection and promoter methylation of secreted coiled-coil-related protein (SFRPs) genes. Methods Seventy-two specimens of gastric mucosal lesions were collected from the Fourth Hospital of Hebei Medical University from October to November in 2012. There were 40 cases of gastric cancer, 17 cases of dysplasia and 15 cases of chronic gastritis. Methylation-specific polymerase chain reaction (MSP) was used to detect the promoter methylation of SFRPs in 72 gastric mucosa tissues, and H.pylori infection was detected by immunohistochemistry. Results The promoter methylation of SFRP1, SFRP2, SFRP4 and SFRP5 genes was found to be 34 (47.2%), 36 (50.0%), 27 (37.5%) and 30 (41.7% . The methylation rates of SFRP1, SFRP2, SFRP4 and SFRP5 gene promoters were 29.4% (5/17), 35.3% (6/17), 11.8% (2/17), 23.5% (4/17) ) And gastric cancer group (72.5% (29/40), 75.0% (30/40), 62.5% (25/40), 65.0% (26/40) 0.05). Gastric cancer group was higher than dysplasia group (P <0.05). The infection rate of H.pylori in 72 specimens was 30.6% (22/72), of which H.pylori infection rate was 76.5% (13/17) and 33.3% (5/15) in dysplasia group and chronic gastritis group In gastric cancer group (10.0% (4/40)] (P <0.05), dysplasia group was higher than that in chronic gastritis group (P <0.05). Spearman rank correlation analysis showed that H.pylori infection was non-linear related to SFRPs promoter methylation (r s = 0.264, P> 0.05). Conclusion The promoter methylation of SFRPs is one of the important factors in gastric carcinogenesis. H.pylori infection is also an important factor in the development of gastric cancer, but the infection of H.pylori is not related to the promoter methylation of SFRPs.