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目的探索在超声监测下,建立兔睾丸缺血再灌注损伤及其不同处理模型。方法选择雄性新西兰大白兔25只,随机分5组:正常对照组A、缺血再灌注组B及不同处理组。在造模过程中,使用超声监测下调节无创血管夹,观察睾丸血流变化;术后3d取睾丸组织,检测MDA、PC、NO、SOD、MPO、GSH-Px表达水平,余组织样本进行HE染色和细胞凋亡检测并Johnsen′s评分,并检测Bcl-2、Bax表达水平。结果 B组术侧与其健侧、A组及不同处理组术侧相比,MDA、PC、NO、SOD、MPO、GSH-Px差异均有统计学意义(P<0.01);病理结果显示:B组术侧睾丸生精细胞和结构受损严重,Johnsen评分显著降低(P<0.01);不同处理组受损程度均改善,评分显著增加(P<0.01),且无统计学差异。B组术侧Bcl-2/Bax比值显著降低(P<0.01),不同处理组其比值明显增加并趋近正常水平(P<0.01),且无统计学差异。结论超声监测下使用无创血管夹制作兔睾丸缺血再灌注损伤及不同处理模型稳定。
Objective To explore the establishment of rabbit testis ischemia-reperfusion injury and its different treatment models under ultrasound monitoring. Methods Twenty-five male New Zealand white rabbits were randomly divided into five groups: normal control group A, ischemia-reperfusion group B and different treatment groups. During the process of modeling, noninvasive vascular clamp was used to monitor the changes of testicular blood flow under ultrasonic monitoring. Testicular tissues were taken after 3 days to detect the expression of MDA, PC, NO, SOD, MPO and GSH-Px, Staining and apoptosis detection and Johnsen’s score, and detection of Bcl-2, Bax expression levels. Results The difference of MDA, PC, NO, SOD, MPO and GSH-Px in group B was statistically significant (P <0.01) compared with that in contralateral group, group A and different treatment groups. The pathological results showed that B The spermatogenic cells and the structure of the testis were severely damaged, and the Johnsen score was significantly lower (P <0.01). The degree of damage was improved and the score was significantly increased (P <0.01) in different treatment groups with no significant difference. The ratio of Bcl-2 / Bax in group B was significantly lower than that in group B (P <0.01). The ratio of Bcl-2 / Bax in group B was significantly increased and approached the normal level (P <0.01), with no significant difference. Conclusion Noninvasive vascular clips under ultrasonic monitoring were used to make rabbit testis ischemia-reperfusion injury and the different treatment models were stable.