【摘 要】
：
Fluorescent probes have been widely employed in biological imaging and sensing. However, it is always a challenge to design probes with high sensitivity. In thi
【机 构】
：
State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineer
论文部分内容阅读
Fluorescent probes have been widely employed in biological imaging and sensing. However, it is always a challenge to design probes with high sensitivity. In this work, based on rhodamine skeleton, we developed a general strategy to construct sensitivity-enhanced fluorescent probe with the help of theoretical calculation for the first time. As a proof of concept, we synthesized a series of HOCl probes. Experiment results showed that with the C-9 of pyronin moiety of rhodamine stabilized by an electron donor group, probe DQF-S exhibited an importantly enhanced sensitivity (LOD: 0.2 nmol/L) towards HOCl together with fast response time (650 nm) and large Stokes shift. Bioimaging studies indicated that DQF-S can not only effectively detect basal HOCl in various types of cells, but also be successfully applied to image tumor tissue in vivo. These results demonstrate the potential of our design as a useful strategy to develop excellent fluorescent probes for bioimaging.
其他文献
水手的儿子 本世纪初,在南中国江海水面上,栖居着一些依水为生的人。冼星海的先辈便是这样的水上居民。到了祖父一代,才移居葡萄牙殖民地——澳门,但是一家人还是靠父亲以水
一种两级路灯照明系统,其前级为基于Sepic电路和反激变换器的单级交直变换器.由于Sepic电路与反激变换器共用一个开关器件,降低了系统的成本,提高了系统的效率和可靠性.同时
Superbuck变换器具有输入输出电流连续的特点,属于高阶变换器拓扑.当主电路参数选择不当的时候会引起变换器小信号模型中存在右半平面(RHP)零点,从而增加控制系统设计的难度.
针对三相-单相矩阵变换器功率解耦方法及闭环控制策略展开研究,提出了一种输出侧含功率解耦单元的三相-单相矩阵变换器拓扑,克服了传统三相-单相矩阵变换器由于输入输出功率
Largely limited by the high dissociation energy of the O--O bond, the photocatalytic molecular oxygen activation is highly challenged, which restrains the appli
有人认为,杨丽萍为跳舞付出得太多。但她觉得这一切很自然。算不上是什么牺牲奉献。因为对她而言,跳舞不是表演、不是职业、不是所谓的艺术,而是一种生活方式、一种同天地相
To realize a fast, easy-operation and precise way using fluorescence probes to quantify analytes is a goal to facilitate detection, especially in situ. Herein,
城市轨道交通的再生制动能量利用是轨道交通节能的重要措施,其中能量转换的核心环节双向DC-DC变换器的合理选择对提高系统的性能和效率、降低系统体积及重量等方面起着重要的
在分析不连续导电模式(DCM)单开关二次型Buck变换器工作原理的基础上,采用非线性控制方法一脉冲序列控制(PT)方法,实现其控制,并研究其稳态及瞬态特性.基于脉冲序列控制的单
在对LCL电路进行深入分析的基础上,提出了LCL电路的一种新型设计原则,它使LCL不但具有滤波器的特性,而且具有电压模式和电流模式,即:在基频下该电路的输出电压(或电流)与负载