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目的通过球囊损伤犬颈动脉模型观察曲尼司特对颈动脉损伤后再狭窄的作用．方法犬被随机分为对照组(n=5)及曲尼司特干预组(50 mg/kg,BID,n=4),颈总动脉损伤前2周及术后4周进行分组干预。通过测定血浆血管紧张素(Ang)I及AngII水平、糜酶(chymase) mRNA表达水平、颈动脉PCNA(细胞增殖核抗原)阳性率及颈动脉各层厚度观察曲尼司特对犬颈动脉损伤后狭窄的作用。结果两组血浆Ang I、Ang II水平及颈动脉外／中膜厚度的差异无统计学意义(P值均>0．05),但曲尼司特干预组较对照组的chymase mRNA表达(A值分别为0．425±0．114比0．708±0．083)、颈动脉各层PCNA阳性率(内膜:0．45±0．05比0．57±0．12,中膜: 0．54±0．05比0．61±0．02,外膜:0．25±0．10比0．36±0．08)及颈动脉内／中膜厚度(0．518±0．044比0．576±0．028)均明显降低(P值<0．05)。结论曲尼司特通过chymase途径对犬颈动脉球囊损伤后的再狭窄具有抑制作用。
Objective To observe the effect of Tranilast on the restenosis after carotid artery injury in dogs by balloon injury. Methods Dogs were randomly divided into control group (n = 5) and tranilast intervention group (50 mg / kg, BID, n = 4), two weeks before carotid artery injury and four weeks after operation. The effects of tranilast on carotid artery injury in dogs were observed by measuring the plasma levels of Ang I and AngII, the expression of chymase mRNA, the positive rate of carotid artery PCNA and the thickness of carotid artery After the narrow effect. Results There was no significant difference in plasma Ang I, Ang II levels and carotid artery outside / medial thickness between the two groups (P> 0.05), but chismase mRNA expression in the tranilast intervention group was significantly higher than that in the control group (0.425 ± 0.114 vs. 0.708 ± 0.083, respectively). The positive rates of PCNA in the carotid arteries (intima: 0.45 ± 0.05 vs 0.57 ± 0.12, in the media: 0.54 ± 0.05 vs. 0.61 ± 0.02, adventitia: 0.25 ± 0.10 vs. 0.36 ± 0.08) and carotid intima / media thickness (0.518 ± 0. 044 vs. 0.576 ± 0.028) (P <0.05). Conclusion Tranilast exerts an inhibitory effect on the restenosis after carotid artery balloon injury in dogs via chymase pathway.