论文部分内容阅读
目的:探讨6种热性中药对大鼠肝脏能量代谢相关因子的影响。方法:6种热性中药(附子、干姜、高良姜、花椒、肉桂和吴茱萸)水提取物分别10.5,8.4,6.0,4.0,3.5,4.2 g.kg-1灌胃大鼠30 d,测定肝脏Na+-K+-ATP酶、Ca2+-ATP酶、琥珀酸脱氢酶(SDH)的活性、肝糖原含量和解偶联蛋白2(UCP2)的mRNA表达水平。结果:6种热性中药均能升高肝组织Na+-K+-ATP酶活性,其中高良姜、花椒、吴茱萸达到了显著水平(P<0.05);6种热性中药有升高肝脏Ca2+-ATP酶活性的趋势,其中高良姜达到了显著水平(P<0.05);6种热性中药均能升高大鼠肝脏SDH活性,除附子组外,其他5种热性中药均达到了极显著水平(P<0.01);6种热性中药均能显著或极显著降低大鼠肝糖原的含量;6种热性中药对大鼠肝脏UCP2 mRNA表达量的影响不明显。结论:热性中药可能通过促进肝糖原的分解、增加SDH酶活性而产生更多ATP,通过增加Na+-K+-ATP酶和Ca2+-ATP酶活性而增加ATP的消耗,从而起到调节肝脏能量代谢的作用。
Objective: To investigate the effects of six kinds of traditional Chinese medicine on the hepatic energy metabolism related factors in rats. Methods: The aqueous extracts of six kinds of heat-sensitive traditional Chinese medicines (aconite, ginger, galangal, pepper, cinnamon and Evodia rutaecarpa) were intragastrically administrated for 30 days at 10.5,8.4,6.0,4.0,3.5,4.2 g.kg-1 respectively. Activity of hepatic Na + -K + -ATPase, Ca2 + -ATPase, and succinate dehydrogenase (SDH), hepatic glycogen content and uncoupling protein 2 (UCP2) mRNA expression. Results: Six kinds of traditional Chinese herbs could increase the activity of Na + -K + -ATP in liver tissue, among which, galangal, Zanthoxylum bungeanum and Fructus Evodiae reached the significant level (P <0.05) (P <0.05). All the 6 kinds of traditional Chinese medicines could increase the activity of SDH in liver of rats, except for the aconite group, the other 5 kinds of traditional Chinese medicine reached the extremely significant level P <0.01). All six kinds of traditional Chinese herbs could significantly or very significantly reduce the content of hepatic glycogen in rats. The effects of six kinds of traditional Chinese herbs on the expression of UCP2 mRNA in the liver were not significant. CONCLUSION: HTM can produce more ATP by promoting the decomposition of glycogen, increasing the activity of SDH and increasing the consumption of ATP by increasing the activity of Na + -K + -ATPase and Ca2 + -ATPase, thereby regulating the liver energy The role of metabolism.