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目的:观察戒毒一号对C57BL/6小鼠的镇静催眠作用,为该药镇静催眠的临床研究提供实验依据.方法:连续15 d灌胃不同剂量的戒毒一号(14.12、56.48 g/kg),以生理盐水组为空白对照,艾司唑仑组为阳性对照.采用小鼠自主活动实验,观察戒毒一号对小鼠自发活动的中枢镇静作用;采用小鼠直接睡眠实验和协同阈上、阈下剂量戊巴比妥钠的睡眠实验,观察戒毒一号催眠作用.结果:高剂量戒毒一号可显著抑制小鼠自主活动(P＜0.01),且没有直接睡眠作用(P＞0.05),能够明显增加阈下剂量戊巴比妥钠诱导小鼠的入睡数及提高入睡率(P＜0.01),对阈上剂量戊巴比妥钠诱导小鼠的睡眠潜伏期及睡眠时间有协同的趋势,但差异无统计学意义(P＞0.05).结论:戒毒一号有一定的镇静催眠作用,且随剂量的升高其镇静和催眠作用均有增强趋势.“,”Objective: To assess the sedative and hypnotic effects of JIEDUYIHAO on C57 BL/6 mice. Methods: The mice were randomly divided into four groups and orally administered with normal saline, estazolam and JIEDUYIHAO at daily doses of 14.12 and 56.48 g/kg respectively. After 15 days of consecutive administration, the autonomic activities test, sleep induced directly test, superthreshold and subthreshold doses of pentobarbital sodium test were used to evaluate the effect of JIEDUYIHAO on sleep behavior in mice. Results: JIEDUYIHAO (56.48 g/kg) could decrease the activities of mice significantly (P＜0.01), and increase the number of mice which were made to sleep by subthreshold dose of pentobarbital sodium (P＜0.01), while there was no directly sleep induced function of JIEDUYIHAO (P＞0.05) and its effect on sleeping time of mice with superthreshold dose of pentobarbital sodium had no significant difference (P＞0.05). Conclusion: JIEDUYIHAO shows dose-dependent sedative and hypnotic effects on mice.