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目的探讨过表达缺氧诱导因子-1α(HIF-1α)对糖尿病心肌缺血再灌注(I/R)损伤的影响,并对其作用机制做初步探讨。方法构建重组HIF-1α真核表达载体,用链脲佐菌素(STZ)诱导建立糖尿病大鼠模型,动物分为正常假手术组(C组)、缺血再灌注组(I/R组)和I/R加HIF-1α诱导组(I/R+HIF-1α组),每组12只大鼠,共36只。检测各组大鼠心肌病理损伤分级、心肌坏死面积、HIF-1α和HO-1的蛋白表达。结果 I/R+HIF-1α组心肌病理损伤分级及心肌坏死面积与I/R组相比明显减少(P<0.05),I/R+HIF-1α组心肌HIF-1α的蛋白表达与I/R组相比明显增加(P<0.05),I/R+HIF-1α组心肌HO-1的蛋白表达与I/R组相比明显增加(P<0.05)。结论过表达HIF-1α,可以调控HO-1的表达,减轻糖尿病大鼠心肌的缺血再灌注损伤,对防治糖尿病大鼠心肌缺血再灌注损伤有一定的作用。
Objective To investigate the effect of over-expression of hypoxia inducible factor-1α (HIF-1α) on myocardial ischemia-reperfusion (I / R) injury in diabetic rats and its mechanism of action. Methods The recombinant HIF-1α eukaryotic expression vector was constructed and induced by streptozotocin (STZ). The animals were divided into normal sham operation group (C group), ischemia reperfusion group (I / R group) And I / R plus HIF-1α induction group (I / R + HIF-1α group), 12 rats in each group. The pathological damage grades, myocardial necrosis area, protein expression of HIF-1αand HO-1 in each group were detected. Results Compared with I / R group, the myocardial pathological grade and area of myocardial necrosis in I / R + HIF-1α group were significantly decreased (P <0.05). The protein expression of HIF-1α in I / R + HIF- (P <0.05). The protein expression of HO-1 in I / R + HIF-1α group was significantly higher than that in I / R group (P <0.05). Conclusion Overexpression of HIF-1α can regulate the expression of HO-1 and alleviate the ischemia-reperfusion injury of myocardium in diabetic rats and play a role in prevention of myocardial ischemia-reperfusion injury in diabetic rats.