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目的:研究SERCA2a mRNA在左心室机械辅助减负荷模型中的表达及其意义,探讨左心室减负荷后心肌分子水平的变化。方法:选用Lewis大鼠,结扎冠状动脉左前降支诱导心力衰竭4周后,移植心力衰竭后的心脏及右肺到受体大鼠腹部(通过供体的升主动脉与受体的降主动脉吻合)。移植2周后评价心脏质量,左心室质量,心肌细胞直径,SERCA2a mRNA的表达情况。结果:心力衰竭后增大的心脏左心室质量、左心室心肌细胞直径于移植后趋于正常;心力衰竭后左心室SERCA2a表达明显降低,移植后趋于正常。结论:左心室减负荷后,心肌逆向重塑伴随着心肌细胞分子水平改变,可能与心肌收缩功能的恢复有关。
Objective: To study the expression and significance of SERCA2a mRNA in left ventricular mechanical-aided reduction model and to explore the change of myocardial molecular level after left ventricular load reduction. Methods: Lewis rats were used to ligate the left anterior descending coronary artery to induce heart failure for 4 weeks. Heart and right lung after heart failure were transplanted to the abdomen of the recipient rats (the ascending aorta and the recipient descending aorta Match). After 2 weeks of transplantation, heart quality, left ventricular mass, myocardial cell diameter and SERCA2a mRNA expression were evaluated. Results: After the heart failure, the left ventricular mass and the diameter of left ventricular myocytes tended to be normal after heart failure. The expression of SERCA2a in left ventricle decreased significantly after heart failure and tended to be normal after transplantation. CONCLUSION: Reverse myocardial remodeling accompanied with change of molecular level of cardiomyocytes may be related to the recovery of myocardial contractile function.