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目的:观察蒙药肉蔻-5味丸对慢性抑郁模型大鼠血清ACTH和CORT含量的影响,并探讨其作用机制。方法:60只雄性SD大鼠,随机分为正常组、模型组、阳性对照组(盐酸氟西汀3.6mg·㎏-1)及蒙药低、中、高剂量组(分别为肉蔻-5味丸0.2g·㎏-1、0.4g·㎏-1、0.8g·㎏-1),每组10只。采用慢性不可预见性应激配合孤养的方法建立抑郁模型,造模的同时连续给药21天,以旷场(Open-field)实验来进行行为学评价,酶联免疫吸附法(ELISA)检测大鼠血清ACTH和CORT含量。结果:模型组大鼠Open-Field实验水平活动得分和垂直活动得分较正常组明显降低,而盐酸氟西汀及肉蔻-5味丸均可不同程度改善模型大鼠抑郁状态。与模型组相比,盐酸氟西汀及肉蔻-5味丸高、中剂量大鼠血清ACTH和CORT含量明显降低(P<0.05或0.01)。结论:肉蔻-5味丸下调慢性抑郁模型大鼠血清ACTH和CORT含量的升高,其机制可能通过调节HPA轴功能来发挥作用。
Objective: To observe the effect of Mongolian medicine Kou Kou 5 Wei Wan on serum ACTH and CORT content in chronic depression model rats and to explore its mechanism. Methods: Sixty male SD rats were randomly divided into normal group, model group, positive control group (Fluoxetine hydrochloride 3.6mg · ㎏-1) and Mongolian low, medium and high dose groups Pills 0.2g · ㎏-1, 0.4g · ㎏-1, 0.8g · ㎏-1), 10 in each group. The depression model was established by chronic unpredictable stress combined with solitary treatment. The models were administered continuously for 21 days. The behavioral evaluation was carried out by Open-field experiment. The enzyme-linked immunosorbent assay (ELISA) Serum levels of ACTH and CORT in rats. Results: The score of Open-Field test and the score of vertical activity in model group were significantly lower than those in normal group. However, fluoxetine hydrochloride and nutmeg-flavored pills all improved the depression of model rats to some extent. Compared with the model group, the levels of serum ACTH and CORT in high and middle dose of fluoxetine hydrochloride and nutmeg-flavoring pill were significantly decreased (P <0.05 or 0.01). CONCLUSION: The decrease of Serum ACTH and CORT levels in rats with chronic depression induced by “Mecome Kou Kou Wei Wei Wan” could be attributed to the regulation of HPA axis function.