论文部分内容阅读
目的:探讨跑台训练与强制性运动康复训练对脑梗死大鼠神经功能恢复的差异。方法:将50只SD雄性大鼠随机分为正常组、假手术组、模型组、跑台训练组(T组)、强制性运动训练组(C组),每组10只,T组及C组于造模后24 h分别进行跑台训练及强制性运动训练,其他各组正常饲养。于术后14天、21天及28天分别进行神经功能缺损评分(NSS评分),随后取脑采用免疫组织化学方法观察脑梗死灶周围巢蛋白(Nestin)、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)、神经胶质酸性蛋白(GFAP)的表达。结果:各亚组同一时间NSS评分组间比较:C组、T组评分高于正常组及假手术组,但明显低于模型组(P<0.05);C组评分明显低于T组(P<0.05);各组内比较差异无统计学意义(P>0.05)。C组、T组Caspase-3的表达明显低于正常组、假手术组及模型组(P<0.05),而Nestin、GFAP的表达高于正常组、假手术组及模型组(P<0.05)。T组Caspase-3的表达明显高于C组(P<0.05),Nestin、GFAP的表达低于C组(P<0.05)。结论:跑台训练与强制性运动康复训练2种方法对脑梗死大鼠神经功能恢复均有效;强制性运动的效果优于跑台训练。
Objective: To explore the difference of neurological function recovery between treadmill training and compulsory exercise rehabilitation training in rats with cerebral infarction. Methods: 50 SD male rats were randomly divided into normal group, sham operation group, model group, treadmill training group (T group), mandatory exercise training group (C group), 10 rats in each group, T group and C Twenty-four hours after modeling, treadmill training and mandatory exercise training were performed respectively. The other groups were reared normally. Neurological deficit scores (NSS scores) were performed at 14 days, 21 days and 28 days after operation, respectively. Then the brain was taken out for immunohistochemistry to detect the expression of Nestin, cysteine aspartyl proteinase -3 (Caspase-3), glial acidic protein (GFAP) expression. Results: The scores of NSS score in each subgroup at the same time were higher in C group and T group than those in normal group and sham operation group, but significantly lower than those in model group (P <0.05); C group was significantly lower than that in T group <0.05). There was no significant difference among the groups (P> 0.05). The expression of Caspase-3 in group C and group T was significantly lower than that in normal group, sham operation group and model group (P <0.05), while the expression of Nestin and GFAP was higher than that in normal group, sham operation group and model group (P <0.05) . The expression of Caspase-3 in T group was significantly higher than that in C group (P <0.05). The expression of Nestin and GFAP was lower than that in C group (P <0.05). Conclusion: Both treadmill training and compulsory exercise rehabilitation training are effective for neurological recovery in rats with cerebral infarction. Mandatory exercise is better than treadmill training.