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目前国外检测端粒酶活性的端粒重复序列扩增方法（ＴＲＡＰ）多采用放射性同位素标记引物或底物，经ＰＣＲ扩增后行放射自显影，不仅费时，而且存在放射性同位素标记和放射性废物处理的问题。我们用Ｋ５６２细胞和人工合成的引物，经ＰＣＲ扩增后用特殊的荧光染料ＳＹＢＲ...
At present, telomere repeat amplification methods (TRAP) for detecting telomerase activity in foreign countries mostly use radioactive isotope-labeled primers or substrates. Autoradiography after PCR amplification is not only time consuming, but also there is radioactive isotope labeling and radioactive waste treatment. The problem. We used K562 cells and artificial primers, after PCR amplification using a special fluorescent dye SYBR...