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采用镉还原法测定了小鼠血清及肝组织内一氧化氮（ＮＯ）的含量，并观察了ｉｐ细菌内毒素（ＬＰＳ）后小鼠血清ＮＯ含量变化。通过检测ＮＯ在体内稳定的代谢产物ＮＯ２－／ＮＯ３－来表示ＮＯ的含量。用本法测定的正常昆明、ＩＣＲ、ＢＡＬＢ／ｃ小鼠血清及肝组织内ＮＯ２－／ＮＯ３－含量分别为３８．７±７．９，４８．８±４．４，４４．２±５．９μｍｏｌ／Ｌ及６４．４±２．２，７９．５±１２．１，７３．３±２９．３ｎｍｏｌ／ｇ；应用本法测定的正常ＩＣＲ小鼠血清及肝组织内ＮＯ２－／ＮＯ３－含量与应用硝酸根还原酶试剂合法测得值基本吻合；ｉｐ１，２，３ｍｇ／ｋｇ细菌内毒素后，ＩＣＲ小鼠血清的ＮＯ２－／ＮＯ３－含量分别上升至１６２．５±４７．８，４９４±１２３．６，６７４．８±２５９．７μｍｏｌ／Ｌ。表明本法测定这些生物样品中ＮＯ的含量是简便、可靠、有效的
The contents of nitric oxide (NO) in serum and liver of mice were determined by cadmium reduction method. The changes of serum NO level in mice were observed after ip bacterial endotoxin (LPS). The NO content is measured by detecting the NO metabolites NO2- / NO3- that are stable in the body. The content of NO2- / NO3- in serum and liver tissue of normal Kunming, ICR, BALB / c mice determined by this method were 38.7 ± 7.9,48.8 ± 4.4,44.2 ± 5, respectively. 9μmol / L and 64.4 ± 2.2,79.5 ± 12.1,73.3 ± 29.3nmol / g. The content of NO2- / NO3- in serum and liver tissue of normal ICR mice And the measured value of nitrate reductase reagent was basically in good agreement. After the bacterial endotoxin ip1,2,3mg / kg, the content of NO2- / NO3- in ICR mouse serum increased to 162.5 ± 47.8 and 494 ± 123.6, 674.8 ± 259.7 μmol / L. It shows that this method is simple, reliable and effective to determine the content of NO in these biological samples