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目的探讨人参皂苷Rg1对吗啡依赖海马神经元胞内游离钙离子浓度(intracellular free Ca2+concentration,[Ca2+]i)的影响。方法采用荧光钙离子指示剂Fluo-3/AM,利用激光扫描共聚焦显微镜观察低剂量(1μmol/L)、中剂量(10μmol/L)、高剂量(100μmol/L)人参皂苷Rg1处理吗啡依赖原代培养大鼠海马神经元后[Ca2+]i的改变。结果低、中、高剂量人参皂苷Rg1单独处理海马神经元前后并没有引起胞内[Ca2+]i的显著改变(P>0.05),而吗啡依赖海马神经元胞内[Ca2+]i较正常海马神经元细胞明显增高(P<0.01)。此外,低、中、高剂量人参皂苷Rg1处理吗啡依赖海马神经元细胞后,均能显著抑制胞内[Ca2+]i的升高(P<0.01),并呈剂量依赖性,Rg1剂量越大,抑制作用越明显(P<0.01)。结论人参皂苷Rg1可以抑制吗啡依赖海马神经元细胞内[Ca2+]i的增高,对吗啡的成瘾性具有明显的拮抗作用,为合理安全有效地应用人参戒毒提供了实验依据。
Objective To investigate the effect of ginsenoside Rg1 on intracellular free calcium concentration ([Ca2+]i) in morphine-dependent hippocampal neurons. Methods Fluorine-containing calcium indicator Fluo-3/AM was used to observe low-dose (1μmol/L), medium-dose (10μmol/L), and high-dose (100μmol/L) ginsenoside Rg1 for morphine-dependent treatment by laser scanning confocal microscopy. Changes of [Ca2+]i after cultured rat hippocampal neurons. RESULTS: Low, middle and high doses of ginsenoside Rg1 alone did not cause significant changes in intracellular [Ca2+]i before and after treatment of hippocampal neurons, whereas intracellular [Ca2+]i in morphine dependent hippocampal neurons was higher than that in normal hippocampal neurons. Metacytosis was significantly higher (P < 0.01). In addition, treatment of morphine-dependent hippocampal neurons with low, medium, and high doses of ginsenoside Rg1 significantly inhibited the increase of intracellular [Ca2+]i (P<0.01) in a dose-dependent manner, with a greater dose of Rg1. The more significant the inhibition was (P<0.01). Conclusion Ginsenoside Rg1 can inhibit the increase of intracellular [Ca2+]i in morphine-dependent hippocampal neurons, and has obvious antagonistic effects on morphine addiction. It provides experimental basis for reasonable, safe and effective application of ginseng detoxification.