【摘 要】
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S-Ribosylhomocysteinase(LuxS) is the key enzyme in the synthetic pathway of a quorum sensing autoinducer AI-2. LuxS from a 2,3-butanediol produced strain Klebis
【机 构】
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School of Life Science and Biotechnology
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S-Ribosylhomocysteinase(LuxS) is the key enzyme in the synthetic pathway of a quorum sensing autoinducer AI-2. LuxS from a 2,3-butanediol produced strain Klebisella pneumoniae CICC 10011 was cloned and characterized. The luxS gene is composed of 540 bp with 172 amino acids encoded. The Km value for S-ribosylhomocysteine(SRH) was (27+1) μmol/L, kcat was (0.112+0.004) s-1 and kcat/Km was 4.4×103 L·mol-1· s-1 at 25℃. LuxS was activated by divalent metal ions, the highest activity was detected with Co2+ form, followed by Mg2+, Ba2+, Mn2+, Fe2+ and Ca2+, and activation constant for Co2+ is (16+2) μmol/L.
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