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研究探讨人肝癌细胞分化过程中生化指标的变化。方法：用丁酸钠（ＳＢ）处理Ｂｅｌ－７４０２人肝癌细胞，测定甲胎蛋白（ＡＦＰ）、γ－谷氨酰转肽酶（γ－ＧＴ）、醛缩酶（ＡＬＤ）、酪氨酸－α－酮戊二酸转氨酶（ＴＡＴ）、碱性磷酸酶（ＡＬＰ）的比活性变化，并观察细胞增殖速度改变。结果：２．５ｍｍｏｌ／Ｌ和５ｍｍｏｌ／ＬＳＢ处理后，Ｂｅｌ－７４０２细胞增殖明显抑制，ＡＦＰ分泌量明显低于对照组细胞（Ｐ＜０．０５或０．０１），反映肝细胞癌变标志酶γ－ＧＴ和ＡＬＤ比活力也明显降低（Ｐ＜０．０１或０．０５），而反映肝细胞分化的酶ＴＡＴ和ＡＬＰ比活力则明显升高（Ｐ＜０．０５或０．０１）。结论：ＳＢ使人肝癌细胞分化过程中ＡＦＰ、γ－ＧＴ、ＡＬＤ、ＴＡＴ、ＡＬＰ均有明显变化，可用这些生化指标来观察肝细胞恶性表型的逆转。
To investigate the changes of biochemical markers in the differentiation of human hepatoma cells. METHODS: Bel-7402 human hepatoma cells were treated with sodium butyrate (SB) to determine alpha fetoprotein (AFP), gamma-glutamyl transpeptidase (γ-GT), aldolase (ALD), tyrosine- The specific activities of α-ketoglutarate transaminase (TAT) and alkaline phosphatase (ALP) were changed, and changes in cell proliferation rates were observed. RESULTS: After treatment with 2.5mmol/L and 5mmol/LSB, the proliferation of Bel-7402 cells was significantly inhibited, and the secretion of AFP was significantly lower than that of the control cells (P<0.05 or 0.01), reflecting the hepatocellular carcinoma marker gamma. The specific activity of GT and ALD was also significantly lower (P<0.01 or 0.05), while the specific activity of TAT and ALP, which reflected hepatocyte differentiation, was significantly higher (P<0.05 or 0.01). CONCLUSIONS: SB significantly changes AFP, γ-GT, ALD, TAT and ALP during the differentiation of human hepatoma cells. These biochemical markers can be used to observe the reversal of hepatocellular malignant phenotype.