论文部分内容阅读
目的了解2014年10月1日—2015年9月31日北京市密云区甲型H3N2流感活动特点,并对病毒血凝素的基因和进化特征进行研究。方法采集哨点医院收集的678份流感样病例样本和50份疫情样本,进行病毒分离培养。选取15株甲型H3N2流感毒株进行血凝素(HA1)基因的扩增和序列测定。采用生物信息软件进行序列比对和进化分析。结果 2014—2015年流感监测季期间,总共分离出87株甲型H3N2毒株。病毒HA1基因出现了Ⅰ、Ⅱ2个明显分支,均有R140I,V186G,F219S氨基酸变异。另外分支Ⅱ的毒株序列均出现A128T、S138A、G142R、N144S、S159Y、N160T、Q311H、R326K的氨基酸变异,涉及A、B 2个抗原决定簇。结论 2014年10月—2015年9月期间,密云区甲型H3N2流感病毒活动活跃,并且其毒株呈现出2个明显不同的进化分支,其中1个分支毒株的血凝素基因发生了改变。
Objective To understand the characteristics of Influenza A (H3N2) influenza activity in Miyun District, Beijing from October 1, 2014 to September 31, 2015 and study the gene and evolution characteristics of the virus hemagglutinin. Methods A total of 678 samples of influenza-like illness collected from sentinel hospitals and 50 samples of the outbreak were collected for virus isolation and culture. Fifteen strains of influenza A (H3N2) were selected to amplify and sequence the hemagglutinin (HA1) gene. Bioinformatics software was used for sequence alignment and evolutionary analysis. Results A total of 87 strains of H3N2 strains were isolated during the 2014-2015 influenza surveillance season. The virus HA1 gene appeared Ⅰ, Ⅱ 2 obvious branches, both R140I, V186G, F219S amino acid variation. In addition, the sequences of A128T, S138A, G142R, N144S, S159Y, N160T, Q311H and R326K were all found in the strain of branch Ⅱ, involving A and B epitopes. Conclusion During the period from October 2014 to September 2015, the Influenza A (H3N2) influenza virus activity in Miyun Region was active, and its strain showed two distinctly different evolutionary branches. The hemagglutinin gene of one branch strain was changed .