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目的：观察１－（２，６－二甲基苯氧基）－２－（３，４－二甲氧基苯乙胺基）丙烷盐酸盐（ＤＤＰＨ）对血管平滑肌细胞增殖的作用及对生长因子ＰＤＧＦ－Ｂ，ｂＦＧＦ及其相关癌基因ｃ－ｓｉｓ，ｃ－ｍｙｃ表达的影响。方法：内皮素建立培养的ＶＳＭＣ增殖模型，氚—胸腺嘧啶核苷（３Ｈ－ＴｄＲ）参入法，流式细胞术，１９９７－０３－２３收稿，１９９７－０４－０４修回＊国家教委优秀年轻教师基金资助项目１同济医院心内科，武汉４３００３０作者简介：熊一力，女，４１岁，博士后钱家庆，男，６３岁，博士生导师，药理教研室主任，中国药理学会常务理事免疫细胞化学及Ｎｏｒｔｈｅｒｎｂｌｏｔ杂交方法测定。结果：ＤＤＰＨ能逆转内皮素所致的３Ｈ－ＴｄＲ参入量增多，阻止ＶＳＭＣ由静止期（Ｇ０／Ｇ１期）进入ＤＮＡ合成期（Ｓ期）和有丝分裂期（Ｇ２／Ｍ期），并能逆转内皮素引起的ＰＤＧＦ－Ｂ，ｂＦＧＦ抗原，ｃ－ｓｉｓ，ｃ－ｍｙｃｍＲＮＡ表达增强。结论：ＤＤＰＨ能抑制ＶＳＭＣ增殖，与生长因子及癌基因调控的分子生物学机制有关。
Objective: To observe the effect of 1- (2,6-dimethylphenoxy) -2- (3,4-dimethoxyphenethyl) propane hydrochloride (DDPH) on the proliferation of vascular smooth muscle cells and Effects of growth factors PDGF-B, bFGF and their related oncogene c-sis, c-myc expression. Methods: Endothelial cells cultured VSMC proliferation model, tritiated - thymidine (3H-TdR) incorporation method, flow cytometry, 1997-03-23 Received, 1997-04-04 Revised * Excellent young State Education Board Teacher Funding Project 1 Department of Cardiology, Tongji Hospital, Wuhan 430030, China. Author: Xiong Yili, female, 41 years old, postdoctor Qian Jiaqing, male, 63 years old, doctoral tutor, director of Department of Pharmacology, executive director of Chinese Pharmacological Society Immunocytochemistry and Northernblot Hybridization method. Results: DDPH could reverse the increase of endothelin-induced 3H-TdR incorporation, prevent the VSMC from entering the DNA synthesis phase (S phase) and mitosis phase (G2 / M phase) from quiescent phase (G0 / G1 phase) Prolonged PDGF-B, bFGF antigen, c-sis, c-mycmRNA expression increased. Conclusion: DDPH can inhibit the proliferation of VSMC, which is related to the molecular biological mechanism of growth factors and oncogenes regulation.