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目的和方法：应用基因工程技术，将ＥＧＦｃＤＮＡ克隆到ｐＬＹ５／ＩＬ２－ＰＥ４０载体的ＥｃｏＲⅠ、ＳｍａⅠ位点之间ＩＬ２基因位置上，再将重组融合基因亚克隆到表达载体ｐＢＶ２２０的ＥｃｏＲⅠ、ＰｓｔⅠ位点上，以探索ＥＧＦ－ＰＥ４０融合基因在大肠杆菌ＤＨ５ａ中的进行表达及其生理功能。结果：ＳＤＳ－聚丙烯酰胺凝胶电泳和Ｗｅｓｔｅｒｎｂｌｏｔ表明：ＥＧＦ－ＰＥ４０融合蛋白获得表达，并且具有ＥＧＦ和ＰＥ４０的免疫学活性。结论：这为进一步研究该融合的蛋白功能和肿瘤治疗打下基础
Objective and Methods: The EGF cDNA was cloned into the IL2 gene of EcoRI and SmaI sites of pLY5 / IL2-PE40 vector using gene engineering technique. The recombinant fusion gene was subcloned into the EcoRI and PstI site of pBV220 In order to explore the EGF-PE40 fusion gene expression in E. coli DH5a and its physiological function. Results: SDS-polyacrylamide gel electrophoresis and Western blot showed that the EGF-PE40 fusion protein was expressed and had the immunological activity of EGF and PE40. Conclusion: This laid the foundation for further study on the function of the fusion protein and tumor therapy