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目的 探讨影响端粒酶活性因素及可能机理。方法 使用ＰＣＲ－ＥＬＩＳＡ法半定量检测端粒酶活性，并进行细胞周期、形态学观察。以人类早幼粒白血病细胞ＨＬ－６０（Ⅰ）和全反式维甲酸（ＡＴＲＡ５μＭ）处理７２ ｈ 的ＨＬ－６０细胞（Ⅱ）为实验对象。再用一定浓度的小鼠腹腔巨噬细胞培养上清液（ＭＣＳ）体外刺激实验细胞。结果 实验细胞Ⅱ在５％ ＭＣＳ作用下，较对照无明显差异；受２０ ％ ＭＣＳ作用４８ ｈ，端粒酶活性可明显上调（Ｐ＜ ０．０５），在第３ 天酶活性下降；在２０％ 、４０％ ＭＣＳ作用下较对照均明显增高（Ｐ＜ ０．０５），但两者（２０％ 与４０％ ）间无明显差异；ＭＣＳ直接作用实验细胞Ⅰ、Ⅱ的端粒酶提取物，未见端粒酶活性明显变化。实验细胞Ⅱ在持续的ＭＣＳ作用下培养２ 周，恢复恶性增殖能力。端粒酶活性变化受细胞群主体分布影响不大。ＭＣＳ作用于具有高端粒酶活性的实验细胞Ⅰ，未发现明显促进作用。无ＭＣＳ的培养体系中，小牛血清浓度不影响实验细胞Ⅰ、Ⅱ的端粒酶活性。结论 ＡＴＲＡ处理的ＨＬ－６０ 细胞的端粒酶是可调节的，未活化的巨噬细胞培养上清液可对其活性进行有效上调，而对ＨＬ－６０ 细胞高水平表达的端粒酶活性影响不大。
Objective To investigate the factors affecting telomerase activity and possible mechanisms. Methods The telomerase activity was detected semi-quantitatively by PCR-ELISA, and cell cycle and morphology were observed. HL-60 cells (II) treated with human promyelocytic leukemia cells HL-60 (I) and all-trans retinoic acid (ATRA 5 μM) for 72 h were used as experimental subjects. The experimental cells were stimulated in vitro with a certain concentration of mouse peritoneal macrophage culture supernatant (MCS). Results Compared with the control, the experimental cells II had no significant difference in 5% MCS. The activity of telomerase was significantly up-regulated by 20% MCS for 48 h (P<0.05), and the enzyme activity decreased on the 3rd day. % and 40% of MCS were significantly higher than controls (P<0.05), but there was no significant difference between the two (20% and 40%); MCS directly affected the telomerase extracts of experimental cells I and II. No significant change in telomerase activity was observed. Experimental cells II were cultured for 2 weeks under sustained MCS to restore malignant proliferation. Changes in telomerase activity were not affected by the distribution of the body of the cell population. MCS acted on experimental cells I with high-end granzyme activity and no significant promotion effect was found. In the MCS-free culture system, calf serum concentration did not affect telomerase activity of experimental cells I and II. Conclusion The telomerase activity of ATRA-treated HL-60 cells can be regulated. The supernatant of cultured unactivated macrophages can effectively up-regulate the activity of telomerase, while the telomerase activity at high levels of HL-60 cells can be affected. Not great.