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目的:探索滇桂艾纳香组培快繁技术体系的最佳培养条件。方法:以滇桂艾纳香的嫩茎段为外植体,采用正交试验设计方法优化增殖培养基,建立组培快繁体系。结果:0.1%Hg Cl2溶液灭菌10 min效果最佳;茎段丛生芽诱导培养基以MS+6-BA 2.0 mg/L+KT 1.0 mg/L+NAA 0.5 mg/L最佳,诱导率达90%;6-BA是影响增殖的主要因素,达显著水平,NAA与KT效应不显著;芽增殖的最适培养基为MS+6-BA 2.5 mg/L+KT 1.5 mg/L+NAA0.2 mg/L,增殖系数为7.12;生根培养基用1/2MS+NAA 0.5 mg/L最好,生根率100%,移栽成活率93.33%。结论:该试验得到的组培快繁技术体系可在短时间内提供大量种苗,并为规模化生产种苗提供技术指导。
Objective: To explore the best culturing conditions for the tissue culture system of A. niger. Methods: The tender stem segments of A. fragrans were used as explants. The orthogonal experiment design method was used to optimize the proliferation medium, and the tissue culture system was established. Results: 0.1% Hg Cl2 solution was the best for 10 min of sterilization. The optimal culture medium for stems bud induction was MS + 6-BA 2.0 mg / L + KT 1.0 mg / L + NAA 0.5 mg / L, 90%; 6-BA is the main factor affecting proliferation, reached a significant level, NAA and KT effect was not significant; buds proliferation optimum medium MS + 6-BA 2.5 mg / L + KT 1.5 mg / L + NAA0. 2 mg / L, and the multiplication coefficient was 7.12. The rooting medium was the best with 1 / 2MS + NAA 0.5 mg / L, the rooting rate was 100% and the survival rate of transplanting was 93.33%. Conclusion: The system of tissue culture and rapid propagation obtained in this experiment can provide a large number of seedlings in a short period of time and provide technical guidance for large-scale production of seedlings.