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目的探讨多环芳烃(PAHs)暴露与新生儿DNA修复酶基因XPD 751位点多态性对新生儿出生体重的影响。方法于2010年使用统一调查问卷调查太原市某医院的229对孕妇及新生儿,检测母亲静脉血清PAHs浓度,采用聚合酶链反应-限制性片段长度多态性(RFLP-PCR)方法检测XPD 751位点多态性。结果单因素、广义线性回归分析发现,未调整混杂因素前,DNA修复酶基因XPD Lys751Gln位点(野生型、杂合/纯合突变型)及母亲PAHs暴露对出生体重的影响均无统计学意义;对孕妇年龄、受教育程度、孕妇体质指数、家庭收入情况、被动吸烟、取暖方式、新生儿性别、孕周等校正后发现,母亲PAHs高暴露组(PAHs>3.17μg/L)的新生儿出生体重低于低暴露组(PAHs≤3.17μg/L),差异有统计学意义(P<0.01)。采用Univariate模型分析孕妇PAHs暴露水平和新生儿DNA修复酶基因XPD Lys751Gln位点(野生型、杂合/纯合突变型)多态性对新生儿出生体重的交互影响发现,母亲处于PAHs高暴露水平、新生儿携带XPD 751突变基因型组的出生体重低于PAHs低暴露野生型组(β=-78.88,P=0.042)。结论 PAHs暴露对新生儿出生体重的影响受其自身修复酶基因型的修饰,提示XPD基因突变可能导致出生体重降低,且存在环境与基因的交互作用。
Objective To investigate the effects of polycyclic aromatic hydrocarbons (PAHs) exposure and neonatal DNA repair enzyme gene XPD 751 polymorphism on newborn birth weight. Methods A total of 229 pairs of pregnant women and newborns in a hospital in Taiyuan were investigated using a unified questionnaire in 2010. The concentrations of PAHs in maternal venous blood samples were detected by polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) to detect XPD 751 Site polymorphism. Results The results of single factor and generalized linear regression analysis showed that there was no significant difference in the birth weight of DNA repair enzyme gene XPD Lys751Gln (wild-type, heterozygous / homozygous mutant) and maternal PAHs before adjustment for confounding factors ; Correction of maternal age, education level, body mass index of pregnant women, family income, passive smoking, heating method, neonatal sex, gestational age and other correction found that high mothers PAHs exposure group (PAHs> 3.17μg / L) The birth weight was lower than the low exposure group (PAHs≤3.17μg / L), the difference was statistically significant (P <0.01). Univariate model was used to analyze the correlation between exposure level of PAHs in pregnant women and XPD Lys751Gln locus (wild-type, heterozygous / homozygous mutant) polymorphisms on neonatal birth weight. The results showed that mothers were at high levels of PAHs exposure The birth weight of neonates with the XPD 751 mutant genotype was lower than that of the wild-type PAHs with low exposure (β = -78.88, P = 0.042). Conclusion The impact of PAH exposure on newborn birth weight is modified by its own repair enzyme genotype, suggesting that the XPD gene mutation may lead to birth weight loss, and there is the interaction between environment and gene.