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为了研究茶氨酸对烟草幼苗生长及生理特性的影响,以云烟87为材料,进行水培试验,对不同茶氨酸浓度(0、0.5、1和5 mmol·L-1)处理下烟草幼苗的生长特征及部分生化指标进行测定。结果表明,茶氨酸会使烟草幼苗根长变短,地上部分、地下部分干重和鲜重、根冠比以及叶绿素含量明显降低;可溶性蛋白、脯氨酸和还原型谷胱甘肽(GSH)含量升高;超氧化物歧化酶(SOD)、过氧化物酶(POD)活性升高,过氧化氢酶(CAT)活性降低,抗氧化酶系统失衡导致H2O2在烟草幼苗体内累积引起膜脂氧化造成损伤,丙二醛(MDA)含量及相对电导率相应的升高进一步说明了上述结论。由此可见,外源施加茶氨酸会使烟草幼苗体内抗氧化系统失衡致使膜脂氧化引发氧化损伤从而抑制其生长。
In order to study the effects of theanine on the growth and physiological characteristics of tobacco seedlings, Yunyan 87 was used to conduct hydroponics experiments. The effects of theanine on tobacco seedlings (0, 0.5, 1 and 5 mmol·L-1) Growth characteristics and some biochemical indicators were determined. The results showed that the theanine could shorten the root length of tobacco seedlings, the dry weight, fresh weight, root / shoot ratio and chlorophyll content of aerial parts decreased obviously. The contents of soluble protein, proline and reduced glutathione ) Content increased; activity of superoxide dismutase (SOD) and peroxidase (POD) increased, catalase (CAT) activity decreased, and imbalance of antioxidant enzyme system led to accumulation of H2O2 in tobacco seedlings, Oxidative damage, the corresponding increase in malondialdehyde (MDA) content and relative conductivity further illustrate the above conclusion. Thus, the application of theanine could make the antioxidation system unbalanced in the tobacco seedlings, resulting in the oxidative damage of the membrane lipid and thus inhibiting its growth.