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以野生型大豆ZYD00006(供体亲本)与黑龙江省主栽品种绥农14(轮回亲本)所构建的回交导入系(832株)为研究材料,利用单因素方差分析方法在11条连锁群上定位到23个QTL位点(P≤0.01),其中4个正效应,19个负效应。导入系群体经过严格的油分含量筛选鉴定,得到13个油分含量性状明显大于轮回亲本的导入系株行和20个油分含量明显小于轮回亲本的导入系植株。利用这33个特殊株行(选择群体)结合随机对照群体,通过基于遗传搭车原理的卡方分析,检测到分布于15个连锁群上的25个与大豆油分含量相关的标记位点,其中11个正效应,14个负效应。两种方法共检测到34个位点,新发现11个位点,两种方法均检测到的有14个位点。
The backcross inbred lines (832 lines) constructed from wild-type soybean ZYD00006 (donor’s parent) and Suinong 14 (reincarnated parent) of Heilongjiang Province were used as experimental materials, and single-factor analysis of variance There were 23 QTL loci (P≤0.01), of which 4 were positive and 19 were negative. The results showed that there were 13 lines with more oil content than that of the recurrent parent lines and 20 lines with less oil content than the recurrent parent lines. Twenty-five marker loci related to soybean oil content were detected by chi-square analysis based on heredity hitchhiking using these 33 special line (selection population) combined with random control population, among which 11 positive effects, 14 negative effects. A total of 34 loci were detected by the two methods, 11 were newly found, and 14 loci were detected by both methods.