论文部分内容阅读
目的探讨碘过量对Wistar大鼠仔鼠大脑锥体细胞的形态学影响。方法将断乳后1个月Wistar大鼠随机分为5组(NI、5HI、10HI、50HI、100HI),饮用不同浓度的碘水,饲养3个月后雌雄合笼,取第二代1、20、60日龄仔鼠,观察仔鼠的脑发育。结果在1日龄仔鼠中,与NI组相比,各碘过量组仔鼠锥体细胞顶树突棘突密度、棘突的分布类型、锥体细胞基树突数、初级树突分枝指数、锥体细胞的最大横截面积、体密度和面数密度均无明显变化(P>0.05);在20日龄仔鼠中,100HI组顶树突棘突密度、锥体细胞的最大横截面积较NI组减小(P<0.05);而在60日龄仔鼠中,50HI组和100HI组与NI组相比都表现出一定程度的脑发育落后,其中以100HI组最为明显(P<0.05)。结论大鼠对碘过量有极强的耐受力,严重碘过量(正常摄入量50倍以上)会影响仔鼠大脑锥体细胞的发育,进而阻碍脑的发育。
Objective To investigate the morphological effects of iodine excess on the pyramidal cells of neonatal Wistar rats. Methods One month after weaning, Wistar rats were randomly divided into 5 groups (NI, 5HI, 10HI, 50HI, 100HI), drinking different concentrations of iodine and water, feeding for 3 months, 20, 60-day-old offspring, observed offspring brain development. Results Compared with NI group, in the 1-day-old pups, the density of spinous process dendrites, the distribution of spinous processes, the number of dendrites in pyramidal cells, the primary dendritic branches Index, the maximum cross-sectional area of cone cells, body density and number density did not change significantly (P> 0.05); 20-day-old pups, 100HI group dendrites top dendritic spine, (P <0.05). However, in 60-day-old offspring, 50HI group and 100HI group showed a certain degree of brain development lag compared with NI group, of which 100HI group was the most obvious (P <0.05). CONCLUSIONS: Rats are extremely tolerant to excessive iodine. Serious iodine excess (above 50 times of normal intake) can affect the development of pyramidal cells in the brains of the offspring, which hinders brain development.