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应用流式细胞术及自动图像分析术,对47例Ⅱ期原发性乳腺癌石蜡包埋瘤组织,进行了DNA倍性及核面积定量分析,并探讨了DNA倍性和核面积的临床意义。结果表明DNA倍性与核面积有密切的关系。DNA倍性和核面积与腋淋巴结转移状况及组织学分级也均有显著性关系,但与肿瘤的大小的关系不明显。二倍体及核平均面积<35μm~2乳腺癌病人术后复发率及无病生存期,均明显好于非二倍体及核平均面积≥35μm~2者。Cox模型多因素生存分析表明,核平均面积和DNA倍性均是影响乳腺癌病人预后的显著性因素。本组结果显示DNA倍性和核面积在估价乳腺癌病人预后上具有重要价值。
Using flow cytometry and automated image analysis, 47 cases of stage Ⅱ primary breast cancer paraffin-embedded tumor tissue were analyzed for DNA ploidy and nuclear area, and discussed the clinical significance of DNA ploidy and nuclear area . The results showed that DNA ploidy and nuclear area are closely related. DNA ploidy and nuclear area and axillary lymph node metastasis and histological grade also had a significant relationship, but the relationship between the size of the tumor is not obvious. Diploid and nuclear average area <35μm ~ 2 breast cancer patients postoperative recurrence and disease-free survival were significantly better than non-diploid and nuclear average area ≥ 35μm ~ 2. Cox model multivariate survival analysis showed that the average nuclear area and DNA ploidy are all the prognostic factors of breast cancer patients significantly. This group of results show that DNA ploidy and nuclear area in the evaluation of the prognosis of breast cancer patients has an important value.