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目的探讨淋巴细胞主动免疫治疗对原因不明复发性流产的疗效及在主动免疫治疗前后患者微量淋巴细胞毒实验淋巴细胞亚群的变化。方法应用荧光染色法和流式细胞术,分别对2009年4月至2010年12月在安徽医科大学第一附属医院行主动免疫治疗的48例原因不明复发性流产患者治疗前后的淋巴细胞坏死率和CD3、CD4、CD8及CD16+CD56+NK细胞的比率进行测定;同时随访其预后。结果 43例成功妊娠至孕12周之后,5例患者在孕12周前流产,主动免疫治疗成功率89.6%(43/48)。治疗后患者淋巴细胞坏死率上升,CD3、CD4和CD16+CD56+NK亚群比率下降,CD8亚群比率上升,差异有统计学意义(P<0.05,P<0.01)。结论淋巴细胞主动免疫治疗对原因不明复发性流产治疗效果满意,CD8亚群的升高可能有利于妊娠。
Objective To investigate the effect of active lymphocyte immunotherapy on unexplained recurrent spontaneous abortion and the changes of experimental lymphocyte subsets in patients before and after active immunotherapy. Methods Fluorescent staining and flow cytometry were used to detect the rate of lymphocyte necrosis in 48 patients with unexplained recurrent spontaneous abortion before and after active immunotherapy at the First Affiliated Hospital of Anhui Medical University from April 2009 to December 2010. And CD3, CD4, CD8 and CD16 + CD56 + NK cell ratio was measured; at the same time, follow-up of its prognosis. Results After 43 cases of successful pregnancy to 12 weeks of pregnancy, 5 patients aborted before 12 weeks of pregnancy. The success rate of active immunotherapy was 89.6% (43/48). After treatment, the rate of lymphocyte necrosis increased, the ratio of CD3, CD4 and CD16 + CD56 + NK subpopulations decreased and the proportion of CD8 subpopulations increased (P <0.05, P <0.01). Conclusions Active immunotherapy of lymphocytes is satisfactory for the treatment of unexplained recurrent spontaneous abortion. Elevated CD8 subpopulation may be beneficial to pregnancy.