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目的探讨不同比例、不同浓度的n-6/n-3多不饱和脂肪酸(PUFA)对3T3-L1脂肪细胞脂联素及过氧化物酶体增殖物激活受体(PPARγ)表达的调节作用。方法用不同比例、不同浓度的n-6/n-3PUFA分别处理已诱导分化成熟的3T3-L1脂肪细胞,用实时定量PCR和Western-Blot法测定各处理组胞内脂联素、PPARγmRNA表达水平以及脂联素蛋白表达水平。结果与空白对照相比,n-6/n-3PUFA为1:1时,在25~200μmol/L范围内时显著促进脂联素mRNA表达;比例为5:1时25、50μmol/L浓度组及比例为10:1时50μmol/L浓度组均显著促进脂联素mRNA表达。比例为20:1及30:1时,各浓度组对脂联素mRNA表达主要起抑制作用。在脂肪酸浓度达到400μmol/L时,n-6/n-3PUFA无论何种构成比均抑制脂联素表达。胞内脂联素蛋白表达与脂联素mRNA表达基本一致。PPARγ表达与脂联素表达呈正相关。结论 n-6/n-3PUFA可能通过PPARγ途径,以剂量依赖方式调节脂联素表达。
Objective To investigate the regulation of adiponectin and peroxisome proliferator - activated receptor (PPARγ) expression in 3T3 - L1 adipocytes by different concentrations of n-6 / n-3 polyunsaturated fatty acids (PUFA). Methods 3T3-L1 adipocytes that had already been induced to differentiate were treated with n-6 / n-3PUFA in different concentrations and concentrations respectively. The expression of adiponectin and PPARγ mRNA in each treatment group was determined by real-time quantitative PCR and Western-Blot And adiponectin protein expression levels. Results When n-6 / n-3 PUFA was 1: 1, the mRNA expression of adiponectin was significantly promoted in the range of 25 ~ 200 μmol / L compared with the blank control; the ratio was 25:50 and 50 μmol / L And the concentration of 50μmol / L at the ratio of 10: 1 significantly promoted adiponectin mRNA expression. At the ratio of 20: 1 and 30: 1, the concentration of adiponectin mRNA expression was mainly inhibited. When fatty acid concentration reached 400 μmol / L, n-6 / n-3 PUFA inhibited the adiponectin expression regardless of the constituent ratio. Intracellular adiponectin protein expression and adiponectin mRNA expression are basically the same. PPARγ expression was positively correlated with adiponectin expression. Conclusion n-6 / n-3 PUFAs may regulate adiponectin expression in a dose-dependent manner through the PPARγ pathway.